Figure 8.

The tunicamycin-induced increase in ATP levels is absent in slt2 mutant cells. YPD-grown overnight seed cultures of the BY4741 wild-type and slt2 mutant strain were refreshed at OD₆₀₀ = 0.1 in the same medium lacking or containing 11.5 mM glucosamine (GlcN) and grown at 30 °C until OD₆₀₀ = 0.3. Aliquots were withdrawn for their immediate analysis (time 0), and cultures were split in two and incubated at 30 °C in the presence (Tn; GlcN+Tn) or absence (Control; GlcN) of 2 μg/mL tunicamycin (Tn) for 4 and 6 h. Data are expressed as nmol of ATP per OD₆₀₀ and represent the mean (±SD) of triplicate assays. ATP kinetics for each strain and condition was repeated at least three times. Different letters represent significant differences at p < 0.05 probability level for each strain and growth condition compared with the corresponding control.