Table 4.
Effect of FBS on the virucidal effects of CAC-717 on IBRV as a representative enveloped virus and BAdV-7 as a representative non-enveloped virus.
| Viruses | FBS Concentration (%) in CAC-717 | CAC-717 + HEPES 1 | DW | Maintenance Medium | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 20 | 30 | 40 | 50 | ||||
| IBRV pH | 12.4 | 12.3 | 12.2 | 12.0 | 11.7 | 11.3 | 10.9 | 7.3 | 5.9 | 7.5 |
| viral titer 2 | ≤0.58 * | ≤0.58 * | ≤0.58 * | 2.21 * ± 0.13 |
6.58 ± 0.25 |
6.33 ± 0.00 |
6.83 ± 0.00 |
7.08 ± 0.25 | 7.08 ± 0.25 | 6.96 ± 0.13 |
| BAdV-7 pH | 12.3 | 12.2 | 12.1 | 11.8 | 11.4 | 11.0 | 10.5 | 7.3 | 5.8 | 7.5 |
| viral titer | ≤0.58 * | ≤0.58 * | 2.46 ** ± 0.13 |
5.83 ± 0.25 |
6.96 ± 0.13 |
6.33 ± 0.25 |
6.46 ± 0.13 |
6.46 ± 0.13 | 6.46 ± 0.38 | 6.21 ± 0.13 |
1 Nine volumes of CAC-717 were pretreated with one volume of 1.0 M HEPES buffer (pH 7.2). 2 Log10 TCID50/25 μL (mean ± SE); * A reduction in viral titer of ≥4 log10 relative to treatment with maintenance medium; ** A reduction in viral titer of ≥3 log10 relative to treatment with maintenance medium.