Table 7.
Virucidal effect of CAC-717 on IBRV after long-term storage at room temperature.
| Lot no. (Date of Storage) |
Storage Period (Years Months) |
Initial pH Value | pH Value after Storage | Log10 TCID50/25 μL (Mean ± SE) | |||||
|---|---|---|---|---|---|---|---|---|---|
| CAC-717 | CAC-717 + HEPES 1 | Maintenance Medium | |||||||
| 2 s | 10 s | 30 s | 1 min | 1 min | 1 min | ||||
| A (7 January 2015) | 6 y 4 m | 12.4 | 12.4 | ≤0.58 * | ≤0.58 * | ≤0.58 * | ≤0.58 * | 7.21 ± 0.13 | 7.08 ± 0.25 |
| B (17 October 2016) | 4 y 7 m | 12.4 | 12.4 | ≤0.58 * | ≤0.58 * | ≤0.58 * | ≤0.58 * | 6.96 ± 0.13 | 6.71 ± 0.13 |
1 Nine volumes of CAC-717 were pretreated with one volume of 1.0 M HEPES buffer (pH 7.2). * A reduction in viral titer of ≥4 log10 relative to treatment with maintenance medium.