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Quantification of type I collagen α-chains secreted from ZF4 cells. Pepsinized collagen was purified from the culture media through salt precipitation. The samples were digested with trypsin after adding stable isotope-labeled synthetic peptides and heat denatured. The amount of each α-chain of type I collagen was determined through LC-MS analysis of marker peptides. Values are means ± SD (n = 3). Differences of means were assessed by one-way ANOVA followed by Tukey’s post hoc test.
