Figure 5.

Thermal melting curves for collagen triple helix obtained by CD spectrometry. ZF4 cells were cultured for 3 days at the temperatures indicated. After the culture media were treated with pepsin at 4 °C, collagen was isolated by salt precipitation. CD signal was measured at 221 nm with increasing temperature (0.25 °C/min). The melting temperature (Tm) was set to the temperature at which the fraction folded was 0.5.