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. 2022 Jan 28;10(2):315. doi: 10.3390/microorganisms10020315

Figure 2.

Figure 2

(A) Antimicrobial activity of P. protegens FJKB0103 and its mutants. For antibacterial assays, 5 μL of overnight culture of P. protegens FJKB0103 and its mutants were dropped onto the PDA plates center. After inoculating at 28 °C for 2 days, the LB mixed with 10% saturated S. aureus Sta24-1 was poured on the PDA plates, inoculated at 37 °C for 12 h. (B) HPLC analysis of 2,4-DAPG production of P. protegens FJKB0103 and its mutants in KB medium. FJKB0103, the wild-type strain of P. protegens FJKB0103; ∆phlD, the phlD gene deleted mutant of P. protegens FJKB0103; ∆phlD-C, the complementary strain of ∆phlD with pRK-phlACBD.