Figure 2.
AgNMS-induced multicaspase activation on FaDu cells after 24 and 48 h exposure. Results expressed as mean (%) ± standard deviation from duplicates of 1 independent experiment. Treatment: control = untreated cells; AgNMS = silver nimesulide complex at 100 µM, after 24 and 48 h exposure. Cell subpopulation (%) for multicaspase activation: Q1 = FAM (−)/7-AAD (−); Q2 = FAM (+)/7-AAD (−); Q3 = FAM (+)/7-AAD (+); Q4 = FAM (−)/7-AAD (+); FAM: carboxyfluoresceinfluorochrome. Statistical analysis: two-way ANOVA followed by Tukey’s test (** p < 0.01, *** p < 0.001 comparing to control group).