Figure 4.

Representative photographs and microphotographs of male Balb/c mice treated with AgNMS complex in a topical adhesive based on bacterial cellulose membrane (AgNMS@BCM). Macroscopic findings: (A) skin with tumor before starting treatment with AgNMS@BCM, (B) skin with reduction of the tumor size after 21 days of treatment (day 21), and (C) skin 21 days after the end of treatment with AgNMS@BCM in a topical adhesive (day 42) showing complete remission of the tumor. Microscopic findings: (D) normal skin with epidermis (arrow) and dermis (arrowhead) without changes (HE, 400×), (E) verrucous cell carcinoma before treatment with clusters of tumor cells invading the dermis (arrows) (HE, 400×), and (F) skin after treatment with metal complex with areas of collagen fibrosis in the dermis (arrows) with regression of tumor cells (HE, 100×).