Figure 6.

Antiviral activity of the four hits against MERS-CoV S pv, the SARS-CoV-2 S pv D614G mutant, and the SARS-CoV-2 S pv K417N/E484K/N501Y/D614G mutant. (A–C) Dose-dependent curves of both the four hits against MERS-CoV-S pv (A), SARS-CoV-2 S pv D614G (B), and the SARS-CoV-2 S pv K417N/E484K/N501Y/D614G mutant. (D) The IC₅₀ of the four hits for inhibition against the MERS-CoV-S pv and variant pv. Caco-2 cells were seeded at a density of 2.4 × 10⁴ cells per well in 96-well plates. After overnight incubation, the cells were treated with the hits; 1 h later, the cells were infected with MERS-CoV-S pv, SARS-CoV-2 S pv D614G, and SARS-CoV-2 S pv K417N/E484K/N501Y/D614G. The infected cells were lysed 23 h later, and the luciferase activities were measured. Data were presented as the means ± SD from three independent experiments.