Table 7.
qPCR primers and probes used in the identification of Fusarium species and P. verrucosum.
| Genotype/Gene | Primer/Probe | Sequence (5′-3′) | Regression Equation, Efficiency of qPCR (E) | References |
|---|---|---|---|---|
| F. avenaceum/F. tricinctum | Avetric f | 5′-AGCAGTCGAGTTCGTCAACAGA-3′ | y = −3.35x + 37.21 | [80] |
| Esyn1 | Avetric r | 5′-GGCYTTTCCTGCGAACTTG-3′ | E = 98.5 | |
| Avetric probe | FAM—CCGTCGAGTCCTCT—MGB | R2 = 0.99 | ||
| F. culmorum | FculC561 fwd | 5′-CACCGTCATTGGTATGTTGTCACT-3′ | y = −3.49x + 35.45 | [81] |
| EF1α | FculC614 rev | 5′-CGGGAGCGTCTGATAGTCG-3′ | E = 93.6 R2 = 0.98 |
|
| F. graminearum | FgramB379 fwd | 5′-CCATTCCCTGGGCGCT-3′ | y = −3.29x + 33.32 | [81] |
| EF1α | FgramB411 rev | 5′-CCTATTGACAGGTGGTTAGTGACTGG-3′ | E = 100 R2 = 0.97 |
|
| F. poae | Poae f | 5′-GCGGCCGCTTTTGTCA-3′ | y = −3.2x + 33.85 | [80] |
| Esyn1 | Poae r | 5′-GCCTTTCCAGCAAGAGATGGT-3′ | E = 99.8 | |
| Poae probe | FAM—AAAGCGGTCGAGTCTG—MGB | R2 = 0.99 | ||
| P. verrucosum | rRNA forward | 5′-TAAGGTGCCGGAATACACGCTCAT-3′ | y = −3.53x + 22.27 | [82] |
| rRNA | rRNA reverse | 5′-TAGTTCATTCGGCCCGTGAGTTGT-3′ | E = 92.7 | |
| PV rRNA-Probe | Fam-TCTAGACAGCCCGACGGTGGCCATGGAAGT-Tamra | R2 = 0.99 |