Figure 2.

Time–kill curves of the 16-fold MIC of HY-133 applied at multiple time points against S. aureus strain ATCC 29213. Plot shows mean values for log₁₀ of the numbers of CFU/mL versus time. The threshold (dotted line) implicates the ≥3−log₁₀ decrease in CFU/mL. Time–kill curves were performed in triplicate (mean ± standard deviation). A culture of S. aureus strain ATCC 29213 without HY-133 was always included as a growth control (dashed line). The times given in the legend show the time points of HY-133 application.