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. 2022 Feb 15;10(2):445. doi: 10.3390/microorganisms10020445

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Time–kill curves of the 16-fold MIC of HY-133 applied against S. aureus strain ATCC 29213 subsequent to bacteria-free HY-133 pre-incubation (exposure times of 0.25 h to 2 h). Plot shows mean values for log₁₀ of the numbers of CFU/mL versus time. The threshold (dotted line) implicates the ≥3−log₁₀ decrease in CFU/mL. Time–kill curves were performed in triplicate (mean ± standard deviation). Cultures of S. aureus strain ATCC 29213 (i) with freshly applied HY-133 and (ii) without HY-133 were included as positive and growth control, respectively. PC, positive control (fresh HY-133); pre-inc., pre-incubation of 16-fold MIC of HY-133 in CAMHB.