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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Virology. 2022 Jan 7;567:65–76. doi: 10.1016/j.virol.2021.12.010

Figure 4. UBR4 associates with RVFV Gn protein.

Figure 4.

A-B) Huh-7 cells were infected with either the parental MP12, V5Gn105 (panel A only), or V5Gn229 virus at a MOI 1. Mock infection control refers to media alone. At 24 hpi, cells were harvested and whole cell lysates were generated. Immunoprecipitation using V5 epitope or Gn antibodies was performed and probed by western blot for UBR4 (panel B only) and Gn proteins using either an antibody raised against Gn or V5. Fifty micrograms of whole cell lysates were used as input controls.