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Immunofluorescence staining for basement membrane components in frozen soleus muscle sections from wild-type (left panels) and nidogen 1-deficient (right panels) animals. The primary polyclonal antibodies used were against nidogen 1 (A and E), nidogen 2 (B and F), laminin 1 (C and G), and perlecan (D and H). Bar, 50 μm.
