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Immunogold histochemistry with antibodies against nidogen 2 on sections of soleus muscle from 6-week-old wild-type (A) and nidogen 1-deficient (B) animals. Note the sparse staining for nidogen 2 in the wild-type muscle basement membrane (arrows) and the strong staining within the basement membrane (arrows) and the extracellular matrix of the endomysium in the nidogen 1-deficient animals. my, myocyte; m, mitochondrion. Bars, 0.32 μm.
