Table 2.
Mitochondrial ATPase activity and oligomycin sensitivity of transformants.
| ATPase Activity (μmol/mg/min) | ||||
|---|---|---|---|---|
| Strain | % ρ+ | −Oligomycin | +Oligomycin | Inhibition (%) |
| aW303 | >99 | 4.16 | 0.78 | 81.3 |
| aΔATP23 | 25 | 1.58 | 1.35 | 16.5 |
| aΔATP23 + ATP23(i) | >99 | 4.01 | 0.74 | 81.5 |
| aΔATP23 + COX1-HA (T1121T) (e) | 27 | 1.77 | 1.43 | 19.2 |
| aΔATP23 + COX1-HA (T1121G) (e) | 87 | 2.82 | 1.57 | 44.3 |
Mitochondria were prepared from wild type, atp23 null mutant, atp23 null mutant transformed with hybrid wild-type COX1-HA (T1121T) plasmid or mutant COX1-HA (T1121G) plasmid grown in YPGal medium. ATPase activity was measured at 37 °C.