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. 2022 Feb 20;23(4):2342. doi: 10.3390/ijms23042342

Figure 1.

Figure 1

IL-17 and TNF-α stimulate CD70 expression in RA FLS and OA FLS. (A) RA FLS (n = 3; white bar) and OA FLS (n = 4; grey bar) were cultured with or without IL-17 (5 ng/mL) and TNF-α (5 ng/mL) for 24 h. CD70 mRNA levels were quantified using RT-PCR and β-actin as a housekeeping gene. Statistical differences between groups were determined using a Mann–Whitney test. (B) RA FLS and OA FLS were stimulated with or without IL-17 (5 ng/mL) and TNF-α (5 ng/mL) for 24 h. (C) RA FLS (n = 14; white bar) and OA FLS (n = 4; grey bar) were stimulated with or without IL-17 (5 ng/mL) and TNF-α (5 ng/mL) for 24 h. To analyze the surface expressions of CD70, flow cytometry was performed using PE-conjugated anti-CD70. Average CD70 expression is displayed as fold change of the mean fluorescence intensity (MFI) compared to control. Data are presented as mean ± standard deviation. Statistical analysis was performed by using a Mann–Whitney test (RA) or a paired t-test (OA). * indicates p < 0.05. *** indicates p < 0.001.