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. 2022 Feb 20;23(4):2342. doi: 10.3390/ijms23042342

Figure 5.

Figure 5

IL-17- and TNF-α-induced migration is attenuated by inhibiting CD70 or HIF-2α. (A) RA FLS were cultured with or without IL-17 (5 ng/mL) and TNF-α (5 ng/mL) for 24 h. To inhibit HIF-2α, 20 μM PT-2385 was added to the culture for 24 h. To inhibit CD70, 1 μg/mL BU69 was added to the culture for 24 h. Following the scratch assay, migrated cells were photographed after cytokine stimulation for 24 h. Magnification is 40×. (B,C) Cell migration of RA FLS (n = 4) was measured using a transwell chamber after 16 h and visualized with crystal violet staining. Following solubilization, the crystal violet dye was quantitated using optical density. Data represent the fold change of the optical density compared to control. Statistical analysis was performed using a Mann–Whitney test. Magnification is 200×. * indicates p < 0.05.