Table 6.
The effects of losartan, mirabegron, and their combination on left ventricular expression of redox enzymes at the mRNA level in DOXO-induced chronic cardiotoxicity at week 9.
| Relative Gene Expression |
Groups | ||||
|---|---|---|---|---|---|
| Control | DOXO | DOXO + L | DOXO + M | DOXO + L + M | |
| Nos1/Ppia | 0.80 ± 0.17 | 0.68 ± 0.07 | 0.7 ± 0.16 | 0.64 ± 0.22 | 0.65 ± 0.05 |
| Nos2/Ppia | 0.48 ± 0.04 | 1.00 ± 0.17 * | 0.75 ± 0.10 * | 0.98 ± 0.2 * | 0.64 ± 0.06 * |
| Nox4/Ppia | 0.96 ± 0.15 | 1.24 ± 0.21 | 1.20 ± 0.39 | 0.24 ± 0.10 * # | 0.38 ± 0.11 # |
| Sod1/Ppia | 1.05 ± 0.05 | 0.97 ± 0.06 | 0.96 ± 0.13 | 0.78 ± 0.15 | 0.62 ± 0.09 * |
| Sod2/Ppia | 0.94 ± 0.05 | 0.88 ± 0.06 | 0.80 ± 0.09 | 0.82 ± 0.09 | 0.51 ± 0.07 * # |
| Sod3/Ppia | 1.21 ± 0.15 | 1.09 ± 0.2 | 0.96 ± 0.21 | 0.54 ± 0.17 * | 0.41 ± 0.09 * # |
| Cat/Ppia | 0.46 ± 0.04 | 0.43 ± 0.03 | 0.48 ± 0.08 | 0.37 ± 0.04 | 0.24 ± 0.04 * # |
Values are presented as mean ± S.E.M., * p < 0.05 vs. control group, # p < 0.05 vs. DOXO-only group (n = 5–9, ANOVA on ranks in the cases of Nos1, Nos2, Nox4, and Sod2 expressions, and one-way ANOVA for the other parameters, Holm-Sidak post hoc test). DOXO: doxorubicin, L: losartan, M: mirabegron, Cat: catalase, Nos1: neuronal nitric oxide synthase, Nos2: inducible nitric oxide synthase, Nox4: NADPH-oxidase isoform 4, Sod1: superoxide dismutase 1 (cytoplasmic), Sod2: superoxide dismutase 2 (mitochondrial), Sod3: superoxide dismutase 3 (extracellular), Ppia: peptidylprolyl isomerase A was used as the house-keeping gene for normalization.