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. Author manuscript; available in PMC: 2022 Feb 25.
Published in final edited form as: J Med Chem. 2020 Nov 17;63(23):14951–14978. doi: 10.1021/acs.jmedchem.0c01464

Figure 5.

Figure 5.

ALARM NMR thiol-reactivity assays. Shown are the 1H-13C HMQC spectra of key 13C-labeled methyl groups of the La antigen protein incubated with DMSO; CPM (N-[4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl]maleimide), positive thiol-reactive compound control; Fluconazole, negative thiol-reactive and aggregation compound control; α-methylene-γ-lactone S17; and α-methylene-γ-lactam 51. Compounds tested at 400 μM final concentrations, with a La antigen protein concentration of 50 μM; spectra for S17 and 51 are from the t = 9 h timepoint, spectra for the DMSO, CPM, and fluconazole spectra are representative.