FIGURE 1.

Experimental inoculation of PFFs/or PBS/tdTomato reporter was validated for ex vivo electrically evoked striatal DA release. Experimental (A) scheme and expression of fluorescent tdTomato in the midbrain (B), in ex vivo striatal slice ipsilateral to the PFFs hemisphere (C, seeded side), and ipsilateral to PBS‐injected hemisphere (D, control side). The FSCV setup and striatal recording site in the dorsolateral striatum (E). Representative traces of electrically evoked DA release with current/voltage (CV) curves in the insets in young mice (2‐month‐old) at 1 m.p.i. (F) and 2 m.p.i. (G) and adult mice (5‐month‐old) at 1 m.p.i. (H) and 2 m.p.i. (I). Evoked DA release ratio in seeded side normalized by their internal control side, at different postseeding time points in young and adult mice (J). Only in the adult mice, DA release shows a significant reduction ([DA]e: Control = 1.852 ± 0.2573, Seeded = 1.424 ± 0.2448, paired Student t test: t(6) = 4.56, **p < 0.01) 2 m.p.i. The slope time constant of DA uptake rate following the evoked release (K), expressed as the percentage in seeded side normalized by their internal control side, was not affected by PFFs inoculation (p > 0.05). n = 5 mice per group, 3–5 technical repetitions per hemisphere in each animal