Skip to main content
. 2021 Nov 21;32(2):e13036. doi: 10.1111/bpa.13036

FIGURE 6.

FIGURE 6

Microglial coverage volume increased in young mice, while adult mice showed limited microglial reactivity. (A) Representative images of IBA1 immunohistochemistry staining (left) and IBA1 merged with TH in SNpc in young (top rows) and adult (bottom rows) mice at different m.p.i. Scale bar: 100 µm. (B) at 1 and 2 m.p.i. revealed a significantly higher IBA1 coverage in young mice (two‐way ANOVA Main Factor Age F (1, 16) = 6.366; *p < 0.05). (C) Representative images of IBA1 and TH immunohistochemistry staining in the striatum of young (top rows) and adult (bottom rows) mice at different m.p.i. Scale bar: 20 µm. Analysis of the microglia coverage volume in SNpc. In the striatum (D), the coverage volume of microglia in dorsolateral regions is higher in young compared with adult mice at 2 m.p.i. (two‐way ANOVA Main Factor Age F (1, 16) = 6.366; *p < 0.05). Reactivity values are expressed as the ratio of microglia coverage volume in the seeded side normalized by each internal control side. n = 5 mice per group, 3 technical repetitions for SNpc, and 9 for striatum per hemisphere in each animal