Figure 2.

Expression of the SLC34A1/PFN3 (sense/antisense) locus in in response to zebularine. The expression of sense (SLC34A1) and antisense transcripts (PFN3) was assessed by RT-qPCR; human testis RNA and RNA extracted from primary kidney cells were used as controls (right panels of (A–C)). (A) Response of the sense transcript to zebularine in HKC-8 cells and (B) HEK293 cells. (C) The most effective dose was used (50 µM) to establish the time course of expression for both the sense (black) and the antisense (grey, only detectable at 24 h) transcripts. Statistical significance was tested by one-way ANOVA followed by Tukey’s test for multiple comparisons, * p < 0.05.