Figure 6.

Transient expression of sense/antisense cassettes in HEK293 cells. Cells were transfected with two doses (50 and 250 ng/mL) of cDNA encoding, either the sense transcript (dark grey) or the antisense transcript (light grey). Expression levels of sense and antisense transcripts were assayed by RT-qPCR to detect potential post-transcriptional stabilizing effects. All values are related to untransfected HEK293 cells. To estimate PCR background, GFP was amplified in all samples (control and shaded area reflecting non-significant changes).