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. 2022 Jan 24;10(2):53. doi: 10.3390/toxics10020053

Table 2.

Differential expression for all genes altered in zebrafish following extended duration 4NP, TCC or TCS exposure starting at 4 h post-fertilization through 5 days post-fertilization and short-term duration starting at 4 dpf through 5 dpf. Significant absolute log2-fold changes (value ≥ 0.75 and adjusted p-value < 0.1) in bold. (ND = no difference in expression). Purple background indicates significant differential expression across all 3 chemicals, yellow across 4NP and TCS, green across 4NP and TCC, and blue across TCC and TCS.

Exposure Concentrations (nM) 0–5 Days Exposure Concentrations (nM) 4–5 Days
Gene Name 4-nonylphenol Triclosan Triclocarban 4-nonylphenol Triclosan Triclocarban
0.1 10 1000 0.1 1 10 0.01 1 100 0.1 10 1000 0.1 1 10 0.01 1 100
Cardiovascular
fbxo32 F-box protein 32 −1.2 −0.8 −0.2 0.4 0.2 1 −0.01 0.5 1.4 0.6 0.2 −0.5 1.1 0.3 0.1 1.7 −1.1 −0.2
tfr1b transferrin receptor 1b 0.4 0.2 0.3 0.1 −0.1 0.2 −0.03 −0.1 −1.2 0.6 0.8 −0.2 0.2 0.1 −0.4 −0.9
myl10 myosin, light chain 10, regulatory −0.1 0.04 −0.3 −0 0.1 −0 −0.2 0.2 −0.1 0.3 0.4 0.8 0.2 0.2 −0.1 −0.8 −0.7 0.1
mat2aa methionine adenosyltransferase II, alpha a −0.1 0.1 0.02 −0 −0 0.4 −0.01 −0.5 −1 −0.1 0.2 0.9 −0.1 0.2 0.3 −0.1 0.2 0.05
hbae1.1 hemoglobin, alpha embryonic 1.1 −0.1 0.01 −0.3 −0.8 −0.3 −0.3 0.1 −0.6 −0.4 −0.4 −0.2 0.9 −0.6 1.1 1 −0.7 −1.6 −0.2
tcp1 t-complex 1 0.3 −0.2 −0.1 −0.9 −0.6 −0.2 0.2 −1.2 −0.2 −0.1 −0.3 0.2 −1 0.3 0.3 −0.3 −0.1 −0.2
hbbe1.2 hemoglobin beta embryonic- 1.2 0.2 0.3 0.04 ND −0.3 −0.4 0.04 −0.1 −0.3 −0 −0.2 0.03 −0.1 1.1 0.4 −0.3 −0.8 −0.8
Neurological
reep3b receptor accessory protein 3b 0.5 0.7 0.2 0.6 0.03 0.2 −0.4 0.2 −0.2 0.5 0.8 0.2 0.8 0.1 0.01 −0.3 −1.4 0.1
syngr3b synaptogyrin 3b −0.03 0.7 0.3 0.9 0.1 0.2 −0.1 0.3 −0.3 0.5 1 0.1 0.9 0.1 −0.1 0.3 −0.2 −0.04
agr2 anterior gradient 2 0.9 0.4 −0.2 −0.5 −0.4 −0.3 0.2 −0.7 −0.8 −0.1 −0.05 0.4 −0.3 0.4 0.05 −0.7 −2.5 −0.1
crygm2d18 crystallin, gamma M2d18 −0.2 −1 −0.1 0.2 0.1 −0.8 0.03 0.04 −0.1 −0.3 0.3 −0.1 −0.6 0.6 0.1 −0.2 −1 0.8
elmo2 engulfment and cell motility 2 −0.2 0.4 −0.1 0.2 −0 0 −0.3 −0 −0.2 0.4 0.8 −0.04 0.4 0.5 −0.1 0.5 1.4 0.02
arr3b arrestin 3b, retinal (X- arrestin) 0.1 0.4 −0.02 −0.1 0.2 0.1 −0.3 −0 −2.6 0.2 0.8 −0.2 0.1 −0.1 −0.2 0.04 −0.4 0.2
opn1sw2 opsin 1 (cone pigments), short-wave-sensitive 2 −0.1 −0.01 0.2 −0.2 −0.3 −0.8 −0.1 −0.1 −0.3 −0.3 −0.4 0.2 −0.4 −0.4 −0.3 −0.8 −1.3 −0.1
si:ch211- 153b23.5 si:ch211-153b23.5 0.9 0.3 −0.2 −1 −0.9 −0.6 0.1 −0.9 0.6 0.7 0.5 1 −0.8 0.8 0.1 −0.4 0.4 0.7
mgst3a microsomal glutathione S- transferase 3a 0.3 −0.1 0.1 −0.9 −0.9 −0.3 0.2 −1.1 −0.5 −0.5 −0.5 −0.1 −1 0.4 0.3 0.02 −0.5 −0.4
rlbp1b retinaldehyde binding protein 1b 0.1 0.1 0.4 −0 −0 0.2 0.2 0.1 −0.8 0.2 −0.1 0.1 0.4 1.5 0.5 −1.1 −0.5 0.04
Metabolic Processes
cel.1 carboxyl ester lipase, tandem duplicate 1 −1.2 −0.7 −0.3 0.6 0.4 0.2 −0.3 0.5 0.4 0.1 −0.3 −0.5 1 0.2 −0.3 0.05 −0.6 −0.5
amy2a amylase alpha 2A −0.9 −0.7 −0.1 0.8 0.4 0.2 −0.1 −0.2 0.5 0.1 −0.2 −0.8 0.8 0.4 −0.2 0.4 0.4 −0.6
prss59.2 serine protease 59, tandem duplicate 2 −0.8 −0.4 0.04 0.3 −0.1 0.2 −0.1 −1 0.1 −0 −0.2 −0.4 0.4 1.1 0.2 0.1 −0.3 −0.4
ela2l elastase 2 like −0.7 −0.3 0.06 0.3 −0.5 −0.6 0.2 −0.2 −0.1 −0.5 −0.9 −0.4 0.5 0.4 −0.9 0.1 −0.8 −0.3
pip5k1cb phosphatidylinositol-4- phosphate 5-kinase, type I, gamma b 0.4 0.7 0.4 0.6 0.3 0 −0.4 0 −0.2 0.5 0.9 0.1 0.8 −0.2 −0.3 0.7 −0.3 0.4
fabp10a fatty acid binding protein 10a, liver basic −0.9 −0.1 −0.1 0.2 0.01 0.3 −0.1 0.04 −0.1 −0.1 −0.02 −0.7 0.4 0.6 0.2 0.4 −0.9 −0.2
zgc:92590 zgc:92590 −0.9 −0.3 −1.1 −0 −0 0 −0.1 −0.3 −1.6 −0.1 0.2 −0.2 0.1 0.8 0.1 −0.03 −1.1 −0.9
si:ch211- 234p6.10 si:ch211-234p6.10 −0.8 −0.03 0.4 0 −0.2 0.2 −0.04 0.1 −0.3 0.4 0.1 −0.6 0.3 0.6 0.2 0.9 0.3 −0.4
lpin1a lipin 1 −0.8 −0.4 −0.4 0.1 −0 0.2 −0.2 0.2 0.9 0.2 −0 −0.3 0.4 0.02 −0.1 0.6 0.2 −0.03
fkbp9 FKBP prolyl isomerase 9 0.8 0.6 −0.02 −0.1 −0.3 0 −0.4 −1.7 −2 −0 1 0.8 −0.2 −0.2 0.04 −0.1 −0.6 −0.1
abcc2 ATP-binding cassette, sub- family C (CFTR/MRP), member 2 −0.4 0.1 −0.1 0.4 1 1 −0.1 0.4 1 0.3 0.2 0.2 0.7 −0.2 0.6 −0.1 0.2 −0.1
plin2 perilipin 2 0.06 −0.2 0.6 −0 0.1 1.1 −0.2 −0.3 1.1 0.8 0.4 0.4 0.3 1 1.1 0.4 −0.6 −0.1
fdps farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferas e, geranyltranstransferase) 0.1 0.4 0.1 −0.1 −0.4 0.4 0.02 −0.1 −1.2 0.03 0.4 0.3 0.5 1 0.6 0.04 0.3 0.1
Immune System
irg1l immunoresponsive gene 1, like 1.5 0.2 0.04 −0.5 −0.2 −0.2 0.2 −0.7 1.8 0.8 0.4 0.6 −0.3 0.8 0.2 −0.3 0.6 1.1
hsp90b1 heat shock protein 90, beta (grp94), member 1 1 0.2 0.3 −0.3 −0.1 −0.2 0.4 −0.9 0.2 0.1 −0.1 0.4 −0.4 −0.4 0.2 −0.3 −0.9 −0.1
ctsl.1 cathepsin L.1 −0.4 0.2 −0.4 0.7 0.3 −0.1 −0.1 −0.2 0.7 0.6 0.5 −1.3 0.8 0.5 −0.5 1 −0.1 −0.4
Extracellular Processes
si:dkey-14d8.6 si:dkey-14d8.6 −1.1 −0.4 0.01 1 0.4 0 −0.2 −0.2 0.5 0.2 −0.2 −0.3 0.9 0.4 −0.5 0.5 −0.2 −0.4
pdk2b pyruvate dehydrogenase kinase 2b −1.1 −0.5 0.004 −0 0.2 0.9 −0.1 0.3 1.2 0.5 −0.2 −0.5 0.3 0.4 0.3 0.8 0.5 −0.6
cpa1 carboxypeptidase A1 (pancreatic) −1.3 −0.9 −0.5 0.1 0.02 0.3 −0.1 −0.6 −0.2 −0.1 −0.3 −0.6 0.3 0.4 0.3 0.9 −0.8 −0.4
Intracellular Processes
si:ch211- 153b23.4 si:ch211-153b23.4 1.2 −0.2 −0.3 −0.9 −0.5 −0.5 0.2 −0.4 1.4 0.8 0.1 0.8 −0.5 1.2 0.5 −0.3 0.05 0.5
mt-tRNA tRNA on mitochondrial genome −0.002 −0.04 −0.05 −0.9 −0.4 −0.6 0.1 0.4 0.5 −0.3 −1.1 −0.4 −0.4 0.2 0.1 −1 −1.9 −0.4
prelid3b PRELI domain containing 3 0.1 0.4 0.3 1 0.4 0.5 −0.4 0.1 −0.5 0.4 0.8 0.1 1.3 0.3 −0.1 0.2 −0.6 0.2
hnrnpa0l heterogeneous nuclear ribonucleoprotein A0, like 0.4 0.2 −0.03 −0.8 −0.5 −0.3 0.001 −1 −0.8 0.2 0.5 1 −0.8 0.5 0.1 −0.1 0.1 −0.02
calcoco1b calcium binding and coiled- coil domain 1b −1 −0.7 −0.3 0.1 0.1 0.4 0.1 0.7 2.2 0.4 −0.2 −1.3 0.7 0.7 0.2 0.9 1.1 −0.1
mknk2b MAPK interacting serine/threonine kinase 2b −0.9 −0.5 −0.2 0 0.1 0.2 0.03 −0.1 1.1 −0 −0.2 −0.4 0.3 0.1 −0 0.6 −0.1 0.1
trim63a tripartite motif containing 63a −0.8 −0.6 −0.2 −0.3 −0.1 0.1 0.2 0.3 0.9 0.04 −0.3 −0.6 0.1 0.2 −0.2 0.5 −1.6 −0.2
si:ch211- 207n23.2 si:ch211-207n23.2 0.9 0.3 0.03 −0.5 −0.4 −0.5 0.3 −0.3 1.5 0.8 0.5 0.2 −0.3 0.5 0.1 0.2 0.3 0.4
si:ch211- 153b23.3 si:ch211-153b23.3 1.2 −0.02 −0.1 −0.1 −0 −0 0.2 0.3 1.9 0.3 0.3 0.5 −0 0.7 0.3 −0.2 0.8 1.3
sult5a1 sulfotransferase family 5A, member 1 1.7 0.7 0.1 −0.1 −0 −0.1 0.1 −0.2 2.2 0.4 0.3 0.2 −0.1 1 0.1 −0.3 0.9 0.7
mt-atp8 ATP synthase 8, mitochondrial −0.5 −0.4 −0.7 −0.4 0.2 0 −0.2 0.8 0.1 −0.7 −1 0.2 −0.3 −0.4 0 −0.5 0.2 −0.1
dgcr8 DGCR8 microprocessor complex subunit 0.1 0.4 0.02 0.2 0.2 0.3 −0.2 0.3 −0.8 0.3 0.8 0.1 0.1 −0.02 0.1 0.1 0.6 0.1
bub3 BUB3 mitotic checkpoint protein 0.2 0.3 −0.04 −0.2 −0.1 −0.1 −0.1 −1.3 −1.1 0.3 0.8 0.7 −0.1 −0.1 −0.1 −0.3 −1 0.5
xpot exportin, tRNA (nuclear export receptor for tRNAs) −0.3 0.2 −0.3 0.1 −0 −0.1 −0.4 −0.8 −1.5 0.3 0.8 0.4 −0 −0.1 −0.2 0 0.4 0.9
si:ch211- 250g4.3 si:ch211-250g4.3 0.1 0.3 0.3 0.7 0.4 0.7 0.1 0.8 1 0.3 0.8 −0.05 0.8 0.5 0.3 0.8 −0.2 −0.02
cebpd CCAAT enhancer binding protein delta −0.1 0.03 0.04 −0.4 0.1 0.6 0.2 −0.2 1.2 0.1 −0.5 −0.8 0 0.4 0.4 0.9 0.01 −0.2
hnrnpa0a heterogeneous nuclear ribonucleoprotein A0a 0.5 0.2 −0.1 −0.5 −0.4 −0.4 −0.1 −0.5 −0.8 0.2 0.4 0.8 −0.8 0 0.02 −0.02 −0.4 −0.3
fkbp5 FKBP prolyl isomerase 5 −0.4 0.1 0.1 0.4 0.7 1 0.2 −0.1 0.2 0.01 −0.5 0.5 0.5 −0.4 ND 0.8 −0.02 −0.03
ddx39ab DEAD (Asp-Glu-Ala-Asp) box polypeptide 39Ab 0.3 −0.1 −0.1 −1 −0.6 −0.4 0.1 −0.6 −0.9 −0.1 −0.2 0.3 −1.2 0.3 0.3 −0.6 −0.9 −0.2
tyrp1b tyrosinase-related protein 1b 0.4 −0.02 0.02 −1 −0.8 −0.4 0.2 −0.2 −1.5 −0.3 −0.4 −0.1 −0.7 0.4 0.2 −1 −0.4 −0.2
hnrnpa0b heterogeneous nuclear ribonucleoprotein A0b 0.5 0.2 0.3 −0.8 −0.5 0.2 0.2 −0.9 −1.3 −0.2 −0.01 0.2 −0.6 0.3 0.4 0.1 0.6 −0.1
phtf2 putative homeodomain transcription factor 2 −0.4 0.2 0.2 0.5 0.2 0.2 −0.2 0.3 −0 0.3 0.3 −0.1 0.9 0.2 0 −0.2 −2.4 0.2
zmp:0000001081 zmp:0000001081 0.2 0.1 0.3 0.7 0.3 0.6 0.2 0.2 1.4 0.2 0.7 −0.3 0.9 0.5 0.2 0.8 0.2 0.2
si:ch211- 113a14.18 si:ch211-113a14.18 −0.003 −0.1 0.02 0.1 0.3 0.1 0.1 0.7 0.1 −0.1 −0.2 −0.3 −0.1 −1.4 −0.5 0.2 2.4 −0.1
zgc:113263 zgc:113263 −0.1 −0.2 0.1 0.2 0.01 0 −0.1 0.2 0.4 0.3 0.2 0.2 0.01 −1.2 −0.2 0.2 1.4 0.1
si:ch211- 132b12.7 si:ch211-132b12.7 0.1 0.4 −0.1 0.2 0 0.7 −0.3 −0.2 −2 −0.2 0.5 −0.1 0.9 1 0.5 0.1 −0.03 −0.1
tm4sf21b transmembrane 4 L six family member 21b −0.02 −0.4 −0.2 −0.1 −0 0.2 0.3 −1 −0.4 −0.5 −0.5 0.1 0.1 1.3 0.6 −0.3 0.2 −0.6
Xenobiotic Signaling
cyp3a65 cytochrome P450, family 3,subfamily A, polypeptide 65 −1 −0.6 −0.1 0.5 1 0.7 0.1 0.6 2 0 −0.5 −0.8 0.5 0.1 0.1 −0.1 −0.6 −0.4
ucp1 uncoupling protein 1 −0.9 −0.4 0.1 0.5 0.1 0.4 −0.1 0.3 1.4 0.1 −0.2 −0.5 0.7 0.9 0.1 0.7 −0.1 −0.5
cyp2k18 cytochrome P450, family 2, subfamily K, polypeptide18 0.3 −0.03 0.2 0.2 2 2.4 −0.1 −0.1 1.9 0.2 −0.2 0.6 0.1 −0.2 0.3 −0.2 2.4 −0.5