FIG. 3.

Mutational analysis of possible posttranslational modification sites in the Cse4p END. (Top) The initiating methionine and the 33-amino-acid END sequence in the N terminus of Cse4-559p. Potential phosphorylation (S or T) or acetylation (K) sites were changed to alanine codons, generating the three new alleles shown. (Bottom left) Plate showing growth phenotypes at 38°C (5 days) of strains carrying the indicated HAn-tagged cse4 mutant alleles as their sole source of Cse4p. All strains grew well at 30°C (data not shown). (Bottom right) Immunoblot showing the expression of cse4-encoded mutant proteins. The values on the left are molecular sizes in kilodaltons.