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. 2000 Sep;20(18):7037–7048. doi: 10.1128/mcb.20.18.7037-7048.2000

FIG. 8.

FIG. 8

Flexible spacing between Cse4p END and HFD. The Cse4p END is spaced 70 amino acids (a.a.) away from the Cse4p HFD in the wild-type protein. This spacing can vary from zero (Cse4-559p) to 309 amino acids (Cse4GFP) without appreciably compromising Cse4p function (indicated by a plus sign); however, Cse4p function was not restored when the END and HFD peptides were expressed separately (indicated by a minus sign).