Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 15;27(4):1294. doi: 10.3390/molecules27041294

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Chemiluminescence signal using the photolysis device in vitro. (A) GSNO standard curve. Plotting the averaged NO values against the initial GSNO concentrations in vitro yielded a strong linear relationship. The associated equation was used to correlate NO release with known concentrations and eventually to estimate the concentration in biological samples. “y” is NO released, and “x” is the initial SNO concentration: y = 1.264x + 20.635. (B) Comparison of chemiluminescent signal of L-CSNO and NaNO₂. We measured NO release from NaNO₂ and L-CSNO dissolved in PBS. NO evolved from increasing concentrations of NaNO₂ was between 0.3 and 0.4 ppb, which is equal to ambient air (N = 3), while L-CSNO gave proportional increase in NO signaling (from 1.5 to 7.9 ppb) with its increased concentration (ANOVA, N = 3, each, p ≤ 0.0001).

Chemiluminescence signal using the photolysis device in vitro. (A) GSNO standard curve. Plotting the averaged NO values against the initial GSNO concentrations in vitro yielded a strong linear relationship. The associated equation was used to correlate NO release with known concentrations and eventually to estimate the concentration in biological samples. “y” is NO released, and “x” is the initial SNO concentration: y = 1.264x + 20.635. (B) Comparison of chemiluminescent signal of L-CSNO and NaNO₂. We measured NO release from NaNO₂ and L-CSNO dissolved in PBS. NO evolved from increasing concentrations of NaNO₂ was between 0.3 and 0.4 ppb, which is equal to ambient air (N = 3), while L-CSNO gave proportional increase in NO signaling (from 1.5 to 7.9 ppb) with its increased concentration (ANOVA, N = 3, each, p ≤ 0.0001).