The cytotoxicity of Levistolide A on Vero and LLC-PK1 cells. Cells were treated with LA (20, 40, 60, 80, or 100 µM); DMSO served as the treatment control. The cell viability value was measured by CCK8 assay 48 h later. The value of each group was normalized by the average value (set at 100%) of the mock-treated group. (A) The cell viability of the Vero cells exposed to LA at designed concentrations. (B) The cell viability of LLC-PK1 cells exposed to LA at designed concentrations. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation.