KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| Dh5α competent E. coli | MCLAB | Cat#DA-196 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA) | Sigma-Aldrich | Cat#E4378 |
| 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid tetrakis (BAPTA) | Santa Cruz Biotechnology | Cat#sc-202076 |
| Dulbecco’s modified Eagle’s medium (DMEM) | GIBCO | Cat#11965-092 |
| fetal bovine serum (FBS) | GIBCO | Cat#16140-071 |
| L-glutamine | GIBCO | Cat#25030-081 |
| penicillin/streptomycin | UCSF Cell Culture Facility | Cat#CCFGK004-153K01 |
| Lipofectamine 2000 | Invitrogen | Cat#11668-019 |
| Matrigel | BD Biosciences | Cat#354234 |
| Critical Commercial Assays | ||
| T7 mMessenger kit | Thermo Fisher Scientific | Cat# AM1344 |
| QuikChange Site-Directed Mutagenesis Kit | Agilent | Cat#200515 |
| Experimental Models: Cell Lines | ||
| HEK293 | ATCC | Cat#CRL-1573 |
| Xenopus oocytes | This study | N/A |
| Experimental Models: Organisms/Strains | ||
| Xenopus Laevis | Nasco | Cat# LM00531 |
| Recombinant DNA | ||
| human CaV1.2/pcDNA3.1 | Findeisen and Minor, 2009 | N/A |
| human CaV1.2 D707A/pcDNA3.1 | Shaya et al., 2014 | N/A |
| human CaV1.2 D707N/pcDNA3.1 | Shaya et al., 2014 | N/A |
| human CaV1.2 D707G/pcDNA3.1 | Shaya et al., 2014 | N/A |
| human CaV1.2 D707E/pcDNA3.1 | This study | N/A |
| human CaV1.2 E1115A/pcDNA3.1 | This study | N/A |
| human CaV1.2 D367A/pcDNA3.1 | This study | N/A |
| human CaV1.2 E1119A/pcDNA3.1 | This study | N/A |
| human CaV1.2 D1420A/pcDNA3.1 | This study | N/A |
| human CaV1.2 G364D/D707G/pcDNA3.1 | This study | N/A |
| human CaV1.2 D707G/G1116D/pcDNA3.1 | This study | N/A |
| human CaV1.2 D707A/A1417D/pcDNA3.1 | This study | N/A |
| rat CaV1.342a/pcDNA6/V5-His ABC | Xu and Lipscombe, 2001 | Addgene#26577 |
| rat CaV1.3 D726A/pcDNA6/V5-His ABC | This study | N/A |
| rat CaV1.3 E1121A/pcDNA6/V5-His ABC | This study | N/A |
| human CaV2.1/pcDNA3.1 | Kim et al., 2008 | N/A |
| human CaV2.1 D667A/pcDNA3.1 | This study | N/A |
| human CaV2.1 E1461A/pcDNA3.1 | This study | N/A |
| rat CaVβ2a/pTracer-CMV2-GFP | Findeisen and Minor, 2009 | N/A |
| rabbit CaVβ3/pSport | This study | N/A |
| rabbit CaVα2δ-1/pcDNA3.1 | Findeisen and Minor, 2009 | N/A |
| sv40 T-antigen/Bluescribe | addgene | Cat#21826 |
| Software and Algorithms | ||
| pCLAMP 9 | Molecular Devices | N/A |
| Clampfit 10 | Molecular Devices | N/A |
| SigmaStat 3.1 | Systat | N/A |