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. 2022 Jan 19;14(2):226. doi: 10.3390/pharmaceutics14020226

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expressions of antioxidant proteins from rat myocardium. HO-1 protein expression elevated significantly to diabetes induced oxidative stress in aged rats. (a,b,d) quantitative band density analyses of HO-1, SOD1, and SOD2 proteins normalized to Beta actin and expressed in the percentage of LEAN (that was considered 100%). (c) A representative Western blot for Beta actin, Hsp32, SOD1, and SOD2 in ZDF rat myocardium treated with BGP-15. All data are presented as the average outcome in a group (mean) ± standard deviation (SD). Statistical analysis was carried out by GraphPad Prism 7.00: D’Agostino and Pearson normality test was used to estimate Gaussian distribution than data were analysed with ordinary one-way ANOVA or Kruskal–Wallis test. The groups were matched by Tukey’s multiple comparisons test.