Table 1.
Dose-response phenotype of CRISPR-edited cell lines
| Compound | 3D7-A10 parent EC50 ± SDa (nM) | Dd2-B2 parent EC50 ± SDa (nM) | Dd2:A597V (CRISPR edited) EC50 ± SDa (nM) | Dd2:T648M (CRISPR edited) EC50 ± SDa (nM) |
|---|---|---|---|---|
| MMV019721 | 400 ± 58 (n = 3) | 400 ± 72 (n = 4) | 6,200 ± 94 (n = 4) | 8,200 ± 1,700 (n = 3) |
| MMV084978 | 110 ± 28 (n = 3) | 150 ± 36 (n = 4) | 43,000 ± 6,800 (n = 3) | 12,000 ± 1,200 (n = 4) |
| Mefloquine | 18 ± 4.6 (n = 5) | 15 ± 5.7 (n = 4) | 31 ± 17 (n = 4) | 20 ± 11 (n = 4) |
| Atovaquone | 0.42 ± 0.05 (n = 3) | 0.30 ± 0.13 (n = 4) | 0.55 ± 0.35 (n = 4) | 0.69 ± 0.38 (n = 4) |
| Dihydroartemisinin | 2.6 ± 1.6 (n = 3) | 4.6 ± 3.0 (n = 5) | 5.6 ± 3.5 (n = 4) | 3.7 ± 3.0 (n = 4) |
| Amodiaquine | 7.4 ± 1.8 (n = 5) | 19 ± 6.0 (n = 4) | 21 ± 2.5 (n = 4) | 20 ± 8.6 (n = 4) |
Data presented as mean ± SD with n bioreplicate assays.
a
SD, standard deviation.