REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
mouse anti-HA	Sigma	Cat#H3663; RRID: AB_262051
rabbit anti-GAPDH	Abcam	Cat#AB9485; RRID: AB_307275
anti-mouse horseradish peroxidase (HRP)-conjugated	Thermo Fisher Scientific	Cat#62-6520; RRID: AB_2533947
anti-rabbit horseradish peroxidase (HRP)-conjugated	Cell Signaling Technology	Cat#7074S; RRID: AB_2099233
Rabbit anti tetra-acetylated H4	Sigma-Aldrich	Cat#06-866; RRID: AB_310270
Rabbit anti-H4K8ac	Cell Signaling Technology	Cat#2594; RRID: AB_2248400
Rabbit anti-H3K9ac	Cell Signaling Technology	Cat#9649; RRID: AB_2248400
Rabbit anti- H3	Cell Signaling Technology	Cat#4499; RRID: AB_10544537
Rabbit anti-PfBiP	(Shonhai et al., 2007)	N/A
800CW goat anti-rabbit IgG	Licor	Cat#926-32211; RRID: AB_621843
anti-mouse Alexa Fluor® 488-conjugated secondary antibody	Cell signaling Technology	Cat#4408S; RRID: AB_10694704
Mouse anti-His antibody	Pierce	Cat#MA1-21315; RRID: AB_557403
Bacterial strains
E. coli DH10Bac	Invitrogen	Cat#10361012
E. coli XL1 cells	Agilent Technologies	Cat#50125058
Chemicals, peptides, and recombinant proteins
SYBR Green I fluorescent dye	Invitrogen	Cat#S7563
MitoTracker Deep Red FM	Life Technologies	Cat#M22426
MMV084978	Medicines for Malaria Venture (Antonova-Koch et al., 2018)	N/A
MMV019721	Medicines for Malaria Venture (Duffy et al., 2017)	N/A
WR99210	Jacobus Pharmaceuticals	CAS 47326-86-3
Anhydrotetracycline hydrochloride	Sigma-Aldrich	Cat#37919; CAS 13803-65-1
Blasticidin S hydrochloride	RPI Corp	Cat#B12150-0.1; CAS 3513-03-9
Trichostatin A	Sigma-Aldrich	Cat#T8552; CAS 58880-19-6
Atovaquone	AK scientific	Cat#G211; CAS 95233-18-4
Chloroquine diphosphate salt	Sigma-Aldrich	Cat# C6628; CAS 50-63-5
Critical commercial assays
Nextera XT kit	Illumina	Cat# FC-131-1024
DNeasy Blood and Tissue Kit	Quiagen	Cat#69506
SuperSignal® West Pico Chemiluminescent substrate	Thermo Fisher Scientific	Cat#PI34080
Expi293™ Expression System Kit	Thermo Fisher Scientific	Cat#A14635
Renilla-Glo(R) Luciferase Assay System	Promega	Cat#E2750
Maxiprep system	Qiagen	Cat#12163
EnzChek™ Phosphate Assay Kit	Thermo Fisher Scientific	Cat#E6646
Deposited data
Whole Genome Sequencing data	NCBI Sequence Read Archive (SRA)	SRA BioProject Accession number: PRJNA684602
Metabolomics Data	National Metabolomics Data Repository (NMDR)	NMDR Project ID: PR001065
Crystal structure of acetyl-CoA synthetase from C. neoformans H99 used for PfAcAS modelling	RCSB Protein Data Bank	PDBID: 5U29
Crystal structure of the adenylation domain of carboxylic acid reductase of Nocardia iowensis in the adenylation conformation used for PfACS11 modelling	(Gahloth et al., 2017)	PDBID: 5MSD
Experimental models: cell lines
H. sapiens Expi293 mammalian cells	Thermofisher	Cat# A14527
S. frugiperda Sf9 insect cells	Novagen	Cat#71104-M
NF54-PfACS11-cKD	This paper	N/A
Dd2-B2	(Cowell et al., 2018)	N/A
NF54-expressing Cas9- and T7 RNA polymerase	(Nasamu et al., 2020)	N/A
NF54-PfAcAS-cKD	This paper	N/A
Dd2-B2-A597V_crispr	This paper	N/A
Dd2-B2-T648M_crispr	This paper	N/A
See Table S2 for additional selected cell lines
Oligonucleotides
Oligos for sequencing of PfAcAS (See Table S7)	Integrated DNA Technologies	N/A
Oligos for CRISPR gene editing and plasmid construction (See Table S7)	ThermoFisher	N/A
Recombinant DNA
pDC2-coCas9-U6.2-hDHFR plasmid	(Lim et al., 2016)	N/A
pDC2-coCas9-gRNA-hDHFR plasmid	(Lim et al., 2016)	N/A
pDC2-coCas9-AcAS-A597V-gRNA1-hDHFR	This paper	N/A
pDC2-coCas9-AcAS-T648M-gRNA1-hDHFR	This paper	N/A
pDC2-coCas9-gRNA2-hDHFR	This paper	N/A
pSN054	(Nasamu et al., 2020)	N/A
AcAS_pSN054	This paper	N/A
pET15b	Novagen	Cat#118754
pET15b-PPT343	This paper	N/A
pFastBac™-HT	Invitrogen	Cat#10584027
pFastBac-PPT358	This paper	N/A
pFastBac-PPT409	This paper	N/A
pFastBac-PPT410	This paper	N/A
Software and algorithms
GATK HaplotypeCaller	(McKenna et al., 2010),	https://gatk.broadinstitute.org/hc/en-us
El-Maven LC/MS data processing software	El-Maven	https://resources.elucidata.io/elmaven
MetaboAnalystR 2.0	(Chong and Xia, 2018; Chong et al., 2019)	https://www.rdocumentation.org/packages/MetaboAnalystR/versions/2.0.0
I-TASSER pipeline	(Yang and Zhang, 2015; Zhang et al., 2017)	https://zhanglab.ccmb.med.umich.edu/I-TASSER/
PyMOL 2.2.3	Schrödinger, Inc.	https://pymol.org/2/
Benchling	Benchling	https://benchling.com/
Image Lab 5.2.0	Bio-Rad	https://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z
Image Studio ver 5.2	Li-Cor	https://www.licor.com/bio/image-studio/
ImageJ	NIH	https://imagej.nih.gov/ij/
Graphpad Prism 8.0	Graphpad Software Inc.	https://www.graphpad.com/scientific-software/prism/