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. Author manuscript; available in PMC: 2022 Feb 25.
Published in final edited form as: Methods Enzymol. 2021 Jul 13;659:243–273. doi: 10.1016/bs.mie.2021.06.014

Fig. 2.

Fig. 2

Autonomously replicating expression vectors permit stable replication and gene expression in T. kodakarensis and E. coli. Constitutive or inducible promoters (purple) drive high levels of ectopic gene expression of sequences cloned into the multiple cloning site (MCS, green). Selections in T. kodakarensis (orange) rely on restorations of prototrophies (agmatine, tryptophan, arginine, uracil), statin resistance (mevinolin, simvastatin) or resistance to toxic nucleoside analogs (6MP, 5-FOA). Expression vectors retain either a pUC or p15A origin (ori) and an antibiotic selection cassette(s) for replication in E. coli (blue): bla (ampicillin resistance); cat (chloramphenicol resistance); neo (kanamycin resistance). The entire pTN1 or pTP2 plasmid sequences (red) permit autonomous replication in T. kodakarensis.