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. Author manuscript; available in PMC: 2022 Feb 25.
Published in final edited form as: Methods Enzymol. 2021 Jul 13;659:243–273. doi: 10.1016/bs.mie.2021.06.014

Table 2.

Selectable markers for T. kodakarensis.

Selection Genetic
background
Selection
requirements
Compatible
strains
Reference(s)
Tryptophan ΔtrpE (ΔTK0254) Minimal medium lacking tryptophan KUW1, KUWH1, KW128 Sato et al. (2003) and Sato et al. (2005)
Simvastatin, mevinolin None Requires overexpression of HMG-CoA reductase (PF1848) Any strain Matsumi, Manabe, Fukui, Atomi, and Imanaka (2007) and Santangelo et al. (2008b)
Agmatine ΔpdaD (ΔTK0149) Minimal or rich medium lacking agmatine supplementation TS559, TS900 Fukuda et al. (2008) and Santangelo et al. (2008b)
Histidine ΔhisD (ΔTK0244) Minimal medium lacking histidine KUWH1 Sato et al. (2005)
6-Methyl purine (6MP) ΔHPRT (ΔTK0664) Minimal medium containing 6MP TS517, TS559, TS900 Santangelo et al. (2010)
Uracil, 5-fluorooratic acid (5-FOA) ΔpyrF (ΔTK2276) Minimal medium lacking uracil and supplemented with 5-FOA, respectively KU216, KW128, TS517, TS559, TS900 Sato et al. (2003)
Citrulline, aspartate + argG (PF0207), + argH (PF0208) Minimal medium lacking citrulline and aspartate Any strain Atomi et al. (2004)