Table 2.
Selection | Genetic background |
Selection requirements |
Compatible strains |
Reference(s) |
---|---|---|---|---|
Tryptophan | ΔtrpE (ΔTK0254) | Minimal medium lacking tryptophan | KUW1, KUWH1, KW128 | Sato et al. (2003) and Sato et al. (2005) |
Simvastatin, mevinolin | None | Requires overexpression of HMG-CoA reductase (PF1848) | Any strain | Matsumi, Manabe, Fukui, Atomi, and Imanaka (2007) and Santangelo et al. (2008b) |
Agmatine | ΔpdaD (ΔTK0149) | Minimal or rich medium lacking agmatine supplementation | TS559, TS900 | Fukuda et al. (2008) and Santangelo et al. (2008b) |
Histidine | ΔhisD (ΔTK0244) | Minimal medium lacking histidine | KUWH1 | Sato et al. (2005) |
6-Methyl purine (6MP) | ΔHPRT (ΔTK0664) | Minimal medium containing 6MP | TS517, TS559, TS900 | Santangelo et al. (2010) |
Uracil, 5-fluorooratic acid (5-FOA) | ΔpyrF (ΔTK2276) | Minimal medium lacking uracil and supplemented with 5-FOA, respectively | KU216, KW128, TS517, TS559, TS900 | Sato et al. (2003) |
Citrulline, aspartate | + argG (PF0207), + argH (PF0208) | Minimal medium lacking citrulline and aspartate | Any strain | Atomi et al. (2004) |