Figure 6.

IgG1+ plasma cell expansion and maturation are more prominent in age-matched participants after wP priming. (A) Heatmap showing over-time changes in memory B-cell and plasma cell subsets in aP-primed (n = 5) or wP-primed (n = 7) adolescents (median values). (B) Over-time distribution of IgG1+ plasma cells representing different maturation stages within total IgG1+ plasma cells. Median values for each population were used to construct the plots. Wilcoxon matched pair signed-rank test followed by Bonferroni correction was used to assess longitudinal differences in percentage of CD20-CD138+ cells in total IgG1+ plasma cells within each cohort. Differences in the percentage CD20-CD138+ cells in total IgG1+ plasma cells between cohorts were assessed using Kruskal–Wallis followed by Dunn’s test but did not yield significant differences. For longitudinal changes, only significant differences compared to baseline are shown. Significant longitudinal differences within a cohort are indicated as *, p < 0.05. Significant differences between cohorts at the same time point are indicated as ##, p ≤ 0.01. d = days after vaccination; aP = acellular pertussis vaccine; wP = whole-cell pertussis vaccine; pre-GC = pre-Germinal Center.