Figure 5.

Influence of the dispersing medium used to perform the DLS analysis on the physico-chemical features of protein-based nanoparticles prepared in 5% w/v glucose solution as a function of the cryoprotectant used during the freeze-drying procedure. DLS analysis was performed at 25 °C in 5% w/v glucose solution or MilliQ water. Gliadin/Zein_B = Mean size and polydispersity index before the lyophilization procedure. Gliadin/Zein_A = Mean size and polydispersity index after the lyophilization procedure. * p < 0.05; ** p < 0.001 with respect to the formulation prepared in MilliQ water before the lyophilization procedure. ^## p < 0.001 with respect to the formulation prepared in glucose solution before the lyophilization procedure n.