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. 2022 Jan 18;14(2):172. doi: 10.3390/v14020172

Table 1.

Primers used to amplify and clone SARS-CoV-2.

Primer ID Sequence 5′-3′
RT1 CTACATAAGCAGCCATTAGATCT
RT2 TTTGTGTAGTACCGGCAGCA
RT3 AGTGTATGCAGGGGGTAATTG
PF1 ATTAAAGGTTTATACCTTCCCAGGTAACAAAC
PR1 TGTGTGGCCAACCTCTTCTGT
PF2 GGGAATGGATAATCTTGCCTGCG
PR2 ACCGGCAGCACAAGACATCT
PF3 AGGGCCAATTCTGCTGTCAAA
PR3 TGCAGGGGGTAATTGAGTTCTGG
PF4 GGCAAACCACGCGAACAAAT
PR4 GTCATTCTCCTAAGAAGCTATTAAAATCACATG
PF3-In CAATTACCCCCTGCACCGGGCCGTCGACCAATTC
PR3-In AGCAGAATTGGCCCTATCGAATATAACTTCGTATAATGTATGCTATACG
F1-T7 TAATACGACTCACTATAGATTAAAGGTTTATACCTTCCCAGGTAACAAAC
F4-R-A-RZ GGGACCATGCCGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTCATTCTCCTAAGAAGCTATTAAAATCACATG
BAC11-RZ-F AATGGCGAATGGGACGCGGCCGCCCGGGCCGTCGA
BAC11-F4-R TGTTCGTTTAGTTGTATCGAATATAACTTCGTATAATGTATGCTATACG
BAC11-F2-R AGATTATCCATTCCCATCGAATATAACTTCGTATAATGTATGCTATACG
F4-F ACAACTAAACGAACAATGTTTGTTTTTCTTG
BAC11-T7-R TAGTGAGTCGTATTAATCGAATATAACTTCGTATAATGTATGCTATACG
F2-F GGGAATGGATAATCTTGCCTGCG
RZ-F GGCCGGCATGGTCCCAGCCT
RZ-R GTCCCATTCGCCATTACCGAG