Table 2.
Primary and secondary antibodies with dilution factors and protocol specifications in the immunohistological studies.
| Antigen | Species | Dilution | Provider | Protocol Specifications |
|---|---|---|---|---|
| TE (Tropoelastin) |
Rabbit (Polyclonal) | 1:750 | Dr. Mecham Washington University | -------------------- |
| FBLN-4 (Fibulin 4) |
Rabbit (Monoclonal) | 1:250 | Abcam (ab125073) | Citrate tampon in heat (pH = 6.0) |
| FBLN-5 (Fibulin 5) |
Rabbit (Polyclonal) | 1:1000 | Abcam (ab202977) | -------------------- |
| FBN-1 (Fibrillin 1) |
Rabbit (Polyclonal) | 1:100 | Abcam (ab53076) | Triton 100 × 0.1% in PBS, 10 min |
| LOX (Lysyl oxidase) |
Rabbit (Polyclonal) | 1:500 | Dr. Sommer CNRS-UMR | Glycine HCl, 30 min RT. 0.2% Hialuronidase, 30 min 42 °C |
| LOXL-1 (Lysyl oxidase-like 1) |
Rabbit (Polyclonal) | 1:250 | Dr. Sommer CNRS-UMR | Glycine HCl, 30 min RT. 0.2% Hialuronidase, 30 min 42 °C |
| IgG (Rabbit) | Mouse polyclonal | 1:1000 | Sigma-Aldrich (RG-96/B5283) |
-------------------- |
Abbreviations: PBS = phosphate buffered saline; RT = room temperature.