Table 3.
Sequences of the primers and their binding temperatures in the RT-qPCR studies.
| Gene | Sequence Fwd (5′→3′) | Sequence Rev (5′→3′) | Temp |
|---|---|---|---|
| GADPH | GGA AGG TGA AGG TCG GAG TCA | GTC ATT GAT GGC AAC AAT ATC CAC T | 60 °C |
| TE (Tropoelastin) |
GTG TAT ACC CAG GTG GCG TG | CGA ACT TTG CTG CTG CTT TAG | 64 °C |
| FBLN-4 (Fibulin 4) |
GTC TTG GAC ATG CCA GGA TTA | TGG AGA TCG TGG GAT AGT TTG | 60 °C |
| FBLN-5 (Fibulin 5) |
GTC TTG GAC ATG CCA GGA ATA | TGG AGA TCG TGG GAT AGT TTG | 58 °C |
| FBN-1 (Fibrillin 1) |
GGT GAA TGT ACA AAC ACA GTC AGC A | ATA GGA ACA GAG CAC AGC TTG TTG A | 60 °C |
| LOX (Lysyl oxidase) |
GCA GAT GTC AGA GAT TAT GAT CA | ATC GCC TGT GGT AGC CAT AGT | 60 °C |
| LOXL-1 (Lysyl oxidase-like 1) |
GCA CCT CTC ATA CCC AGG GC | TGG CAG TCG ATG TCC GCA T | 60 °C |