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. 2022 Feb 18;23(4):2253. doi: 10.3390/ijms23042253

Figure 4.

Figure 4

Western blot to detect exogenous BCKDK (Ab anti-Flag); E1α subunit of BCKDH; phosphorylated form of E1α subunit of BCKDH, and HSP90 as charge control. From left to right: HEK-293 were left untreated, transduced with a transduction control (EmGFP), transduced with wild-type exogenous BCKDK, and transduced with mutated form of BCKDK (p.Thr334del).