FIG. 11.

(A) Karmellae are distinctly different from the structures labeled by Tsc13p-GFP. Karmellae form upon overexpression of an Hmg1p-GFP construct (a, GFP fluorescence); orientation of karmellae localized on the nuclear envelope is always on the opposite site of the vacuole (b, FM4-64 staining). c, DIC transmission image. (B) Enrichment of Tsc13p-GFP to the sites of nucleus-vacuole interaction depends on the presence of vacuolar membranes. pep3 mutants contain vestigial vacuoles (b, FM4-64 staining); localization of Tsc13p-GFP appears dispersed on the surface of the nuclear membrane; polarization of the GFP signal is absent (a). c, DIC transmission image.