FIG. 2.

A mutation in the TSC13 gene suppresses the Ca²⁺ sensitivity of the csg2Δ mutant and confers temperature-sensitive lethality. The elo2Δ and elo3Δ mutations also suppress the Ca²⁺ sensitivity of the csg2Δ mutant, and the tsc13-1 mutation displays synthetic growth phenotypes with the elo2Δ and elo3Δ mutations. The indicated strains (Table 1) were grown in SD medium to an OD₆₀₀ of 0.1 and then diluted 1/100 into the wells of a microtiter plate. The cells were transferred to plates containing the indicated medium with or without 50 mM CaCl₂ (Ca), and the plates were incubated at 26°C for 3 days or 37°C for 2 days.