FIG. 4.
The tsc13-1, elo2Δ, and elo3Δ mutants accumulate high levels of free LCBs. LCBs were extracted from 10 OD600 units of the indicated cells, separated by TLC, and visualized by ninhydrin staining. The LCB standards sphingosine (SPH), dihydrosphingosine (DHS), phytosphingosine (PHS), and 3-ketosphingosine (3-KS) were spotted in lane 1 (positions indicated). The ninhydrin-reactive species that migrate just below DHS and near the origin (indicated by the arrows) are phosphatidylserine (PS) and phosphatidylethanolamine (PE).