FIG. 8.

Tsc13p coimmunoprecipitates with Elo2p and with Elo3p. Microsomes were prepared from strain TDY2058 cells containing no tagged proteins (lanes 5 and 9) or the same cells containing only Myc-tagged Tsc13p (lanes 6 and 10), Myc-tagged Tsc13p and HA-tagged Elo2p (lanes 1, 3, 7, and 11) or Myc-tagged Tsc13p and HA-tagged Elo3p (lanes 2, 4, 8, and 12); 10 μg of total microsomal protein was loaded in lanes 1 and 2. The microsomes were solubilized, and the 100,000 × g supernatant was used for immunoprecipitation experiments using anti-GST (lanes 3 and 4), anti-HA (lanes 5 to 8), or anti-Myc (lanes 9 to 12) antibodies. The immunoprecipitated proteins were separated by SDS-PAGE (12% gel) and analyzed by immunoblotting with HRP-conjugated anti-Myc (top panel) or anti-HA (bottom panel) antibodies.