TABLE 3.
Sequences of oligonucleotides used for constructing Tsc13p-GFP and Opi3p-GFP
| Oligonucleotide | Sequencea |
|---|---|
| pYDL015cGA5→ | 5′-caaaaagtatcataccagaagagcattcttgattccatttgtatttGGAGCAGGTGCTGGTGCTGGTGCTGGAGCA-3′ |
| pYDL015cMX6← | 5′-gctaatatctctttaccttgcatttgggcatgttgcaaacaggaggatcaATCGATGAATTCGAGCTCGTTTAAAC-3′ |
| pYDL015c→ | 5′-gtaacgctaagatccgtgtc-3′ |
| pOPI3GA5→ | 5′-ccttttactgccatgatctacgctaaccgtgataaggccaaaaagaatatgGGAGCAGGTGCTGGTGCTGGTGCTGGAGCA-3′ |
| pOPI3MX6← | 5′-cggtaatagcataggcttctaacattatagaatatatagaaatagagcactcaATCGATGAATTCGAGCTCGTTTAAAC-3′ |
| pOPI3→ | 5′-ggatgagagagtcaccggc-3′ |
| kanMX-reverse← | 5′-ctagcggatctgccggtagag-3′ |
| pGFP4← | 5′-ggtcaatttaccgtaagt-3′ |
a
Lowercase, coding gene sequence; italic, 3′ untranslated region gene sequence; uppercase lightface, GA5 linker sequence (30 bp); uppercase boldface, kanMX6 sequence (26 bp); underlined, stop codon.