Figure 2.

Cloud plot generated in the XCMS web-based application showing positive ion mode retention time versus m/z of features with high fold change and statistical significance between injured (green) and uninjured (red) models. The black traces outline chromatographic retention time on the x-axis and m/z values on the y-axis for each sample. Each bubble in the plot corresponds to one metabolite feature with fold change at or above 1.5 and a p-value at or below 0.05 using a Welch’s t-test. The color and size of each bubble denote the directionality and magnitude of fold change, respectively, with larger bubbles representing larger fold changes. Darker bubbles correspond to features with greater statistical significance. Features with m/z values above 2100 are truncated for ease of visibility.