Skip to main content
. 2022 Feb 8;10(2):257. doi: 10.3390/vaccines10020257

Table 1.

List of CD4+ T cell epitopes and CD8+ T cell epitopes validated from HBV proteins.

Sequence Protein Position Reference HLA Restriction Method to Screen Candidate Epitopes Method to Validate the Candidate Epitopes
MQLFHLCLI Core 1–8 [61] A*0201 Predicted Binding assay; ELISpot; Cytotoxicity assay; CTL assay
KEFGASVEL(L) Core 7–15/16 [62] A*0206, B*4001 Predicted ELISpot; ICS; Binding assay
EFGASVELL Core 8–16 [63] A*0201, A*0207 overlapping ICS; ELISpot
FLPSDFFPS Core 18–26 [64] A*0201 Predicted ICS; Tetramer staining
FLPSDFFPSV Core 18–27 [45,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79] A*02, A*0201, A*0202, A*0203, A*0206, A*6802, A*0301, A*0207 overlapping Immunization of mice; Cytotoxicity assay; CTL assay; Binding assay; Tetramer staining; ELISA
LPSDFFPSV Core 19–27 [74,80,81,82,83] B*3501, B*51, B*5301, B*5401, B*07, B*51, A*0201 overlapping Binding assay; CTL assay; Cytotoxicity assay; Tetramer staining
FFPSIRDLL Core 23–31 [84] A*24 Predicted Tetramer staining
DLLDTASALY Core 39–48 [81] A*0101, A*2902, A*3002 Predicted Binding assay; Immunization of mice; ELISpot
DFFPSIRDL Core 51–59 [85] A*2402 Predicted ELISpot
LCWGELMNL Core 60–68 [86] A*0201 Predicted Stabilization assay; ELISpot assay
ELMNLATWV Core 64–72 [87] A*02 Predicted Binding assay; ELISpot; Cytotoxicity assay
SYVNMNMGL Core 87–95 [88] A*2402 Predicted Binding assay; CTL assay
SYVNTNMGL Core 87–95 [89] A*02 Predicted Tetramer staining
YVNVNMGLK Core 88–96 [63] A*1101 overlapping ICS; ELISpot
MGLKFRQL Core 93–100 [90] A*0201 Predicted Immunization of mice; FACS
LLWFHISCL Core 101–108 [43] A*0201 Predicted Proliferation assay; ICS; Cytotoxicity assay
LWFHISCLTF Core 101–110 [85] A*2402, A*2301 Predicted ELISpot
HISCLTFGR Core 104–112 [91,92] A*33 Predicted Cytotoxicity assay; ICS; Tetramer staining
CLTFGRETV Core 107–115 [93] A*02 Predicted Tetramer staining
EYLVSFGVW Core 117–125 [81,84,88] A*2402, A*2407, A*2301 Predicted Stabilization assay; CTL assay; Cytotoxicity assay; Tetramer staining; Binding assay; Immunization of mice; ELISpot
YLVSFGVWI Core 118–126 [43] A*0201 Predicted Proliferation assay; ICS; Cytotoxicity assay
LVSFGVWIR Core 119–127 [91] A*33 Predicted Stabilization assay; ELISpot; Cytotoxicity assay; Immunization of mice
GLKILQLL Core 123–130 [82] B*08 overlapping ICS; Tetramer staining
AYRPPNAPI Core 131–139 [94] A*0201 Predicted ELISpot; Cytotoxicity assay
LTFGRETVLEN Core 137–147 [95] A*0101, A*02, A*2902, A*3002 Predicted ELISpot
ILSTLPETTV Core 139–148 [75] A*02 Predicted CTL assay
STLPETTVVR Core 141–150 [74,96] A*11, A*6801, A*02 overlapping Binding assay; CTL assay; Cytotoxicity assay; ELISpot
STLPETTVVRR Core 141–151 [17,76,81,92,97] A*31, A*68, A*02, A*0201, A*6801, A*03, A*11, A*3101, A*0201 overlapping Cytotoxicity assay; Immunization of mice; CTL assay; Binding assay; ELISpot
TLPETTVVRR Core 142–151 [63] A*1101 overlapping ICS; ELISpot
GVWIRTPPA Core 152–160 [98] A*0201 Predicted ELISpot
STLPETAVVRR Core 170–180 [9] A*1101 Predicted Proliferation assay; Tetramer staining
RTQSPRRR Core 196–203 [9] A*1101 Predicted Proliferation assay; Tetramer staining
RTQSPRRRR Core 196–204 [9] A*1101 Predicted Proliferation assay; Tetramer staining
RSQSPRRRRSK Core 196–206 [9] A*1101 Predicted Proliferation assay; Tetramer staining
RLCCQLDPA HBx 4–12 [99] A*0201 Predicted Binding assay; ELISpot; Cytotoxicity assay
AYFKDCVFKDW HBx 6–16 [45] A*2402 Predicted ELISA
QLDPARDVL HBx 8–16 [45,65,73,99,100,101] A*0201 Predicted ELISpot
VLCLRPVGA HBx 15–23 [45,99,102] A*0201 Predicted ELISpot
RGRPVSGPF HBx 26–34 [85] A*2402 Predicted ELISpot
PVSGPFGPL HBx 29–37 [100] A*0201 Predicted Immunization of mice; CTL assay; Cytotoxicity assay
AVPADHGAHL HBx 44–53 [100] A*0201 Predicted Immunization of mice; CTL assay; Cytotoxicity assay
HLSLRGLPV HBx 52–60 [65,99,100,101,102,103] A*0201, A*02 Predicted Cytotoxicity assay; Immunization of mice; CTL assay; Binding assay; ELISpot
LPVCAFSSA HBx 58–66 [45] B*0702 Predicted ELISA
AFSSAGPCALRF HBx 62–73 [45] A*2402 Predicted ELISA
ALRFTSARR HBx 70–78 [45] A*0301 Predicted ELISA
ALRFTSARRM HBx 70–79 [100] A*0201 Predicted Immunization of mice; CTL assay; Cytotoxicity assay
NAHQILPKV HBx 84–92 [99] A*0201 Predicted Binding assay; ELISpot; Cytotoxicity assay
(K)VLHKRTLGL HBx 91/92–100 [65,100,102] A*0201 Predicted Cytotoxicity assay; Binding assay; ELISpot; Tetramer staining
VLHKRTLGL HBx 92–100 [99,101,104] A*0201, A*02 Predicted Binding assay; ELISpot; Cytotoxicity assay; Proliferation assay; ELISpot; ICS
TLGLAAMST HBx 97–105 [100] A*0201 Predicted Binding assay; ELISpot; Cytotoxicity assay
GLSAMSTTDL HBx 99–108 [99,100,104] A*0201, A*02 Predicted Binding assay; ELISpot; Cytotoxicity assay
AMSTTDLEA HBx 102–110 [99] A*0201 Predicted Binding assay; ELISpot; Cytotoxicity assay
STTDLEAYFK HBx 104–113 [45] A*1101 Predicted ELISA
DLEAYFKDCL HBx 107–116 [100] A*0201 Predicted Immunization of mice; CTL assay; Cytotoxicity assay
CLFKDWEEL HBx 115–123 [99,100,102] A*0201 Predicted Immunization of mice; CTL assay; Cytotoxicity assay; Binding assay; ELISpot
ELGEEIRLKV HBx 122–131 [100] A*0201 Predicted Immunization of mice; CTL assay; Cytotoxicity assay
EIRLKVFVL HBx 126–134 [100] A*0201 Predicted Immunization of mice; CTL assay; Cytotoxicity assay
VLGGCRHKL HBx 133–141 [99,101] A*0201, A*02 Predicted Binding assay; ELISpot; Cytotoxicity assay; ELISpot
VLGGCRHKL(V) HBx 133–141/142 [98] A*0201 Predicted Immunization of mice; CTL assay; Cytotoxicity assay
LLDDEAGPL Pol 13–21 [105,106] A*0201 Predicted Binding assay; Immunization of mice; CTL assay; Cytotoxicity assay
PLEEELPRL Pol 20–28 [105,106] A*0201 Predicted Binding assay; Immunization of mice; CTL assay; Cytotoxicity assay
DLNLGNLN Pol 40–48 [106] A*0201 Predicted Binding assay; Immunization of mice; CTL assay; Cytotoxicity assay
NLGNLNVSI Pol 42–50 [106] A*0201 Predicted Binding assay; Immunization of mice; CTL assay; Cytotoxicity assay
NVSIPWTHK Pol 47–55 [9,74,81] A*03, A*11, A*6801, A*0301, A*1101 Predicted Stabilization assay; ELISpot; Cytotoxicity assay; Immunization of mice; Proliferation assay; Tetramer staining; Binding assay
KVGNFTGLY Pol 55–63 [45,74] A*0301, A*03, A*11 Predicted Binding assay; CTL assay; Cytotoxicity assay; ELISA
GLYSSTVPV Pol 61–69 [73,105,106] A*0201 Predicted Binding assay; Immunization of mice; CTL assay; Cytotoxicity assay; Tetramer staining
LYSSTVPVF Pol 62–70 [79] A*24 Predicted ELISpot
STVPCFNPK Pol 65–73 [9] A*1101 Predicted Proliferation assay; Tetramer staining
TVPCFNPK Pol 66–73 [9] A*1101 Predicted Proliferation assay; Tetramer staining
PSFPHIHLK Pol 77–85 [9] A*1101 Predicted Proliferation assay; Tetramer staining
QYVGPLTVN Pol 94–102 [85] A*2402 Predicted ELISpot
YLHTLWKAGI Pol 147–156 [65] A*02 Predicted ELISpot assay; Tetramer staining
(H)TLWKAGILYK Pol 149/150–159 [81] A*03 Predicted Binding assay; Immunization of mice; ELISpot
HTLWKAGILYK Pol 149–159 [74,76,98] A*03, A*11, A*3101, A*3301, A*6801, A*02, A*11 Predicted Immunization of mice; Cytotoxicity assay; Binding assay; CTL assay
TLWKAGILY(K) Pol 150–158/159 [74] A*03, A*11 Predicted Binding assay; CTL assay; Cytotoxicity assay
RSASFCGSPY Pol 164–173 [45] A*1101 Predicted ELISA
ASFCGSPYSW Pol 166–175 [45,62,63] A*2402, B*5801 overlapping ELISA; ELISpot; ICS
SFCGSPYSW Pol 167–175 [45] A*2402 Predicted ELISA
ASFCGSPY Pol 166–173 [81,95,107] A*0101, A*2902, A*3002 overlapping Binding assay; Immunization of mice; ELISpot; Tetramer staining
SPYSWEQEL Pol 171–179 [17] A*0201, B*3501 Predicted Tetramer staining
QSSGILSR Pol 200–207 [9] A*1101 Predicted Proliferation assay; Tetramer staining
GILPRSSVGPR Pol 205–215 [9] A*1101 Predicted Proliferation assay; Tetramer staining
CLHQSAVRK Pol 274–282 [45] A*0301, A*1101 Predicted ELISA
KTAYSHLSTSK Pol 283–293 [9] A*1101 Predicted Proliferation assay; Tetramer staining
SSARSQSER Pol 310–318 [9] A*1101 Predicted Proliferation assay; Tetramer staining
CLSLIVNLL Pol 338–346 [65] A*02 Predicted ELISpot assay; Tetramer staining
TPARVTGGV Pol 354–362 [45] B*0702 Predicted ELISA
TPARVTGGVF Pol 354–363 [45] B*0702 Predicted ELISA
RVTGGVFLV Pol 357–365 [45] A*0201 Predicted ELISA
VTGGVFLVDK Pol 358–367 [45] A*1101, A*03 Predicted ELISA
RIPRTPSRV Pol 361–369 [65] A*02 Predicted ELISpot assay; Tetramer staining
TPARVTGGVF Pol 365–374 [74,76,108] B*0702, B*3501, A*03, B*07, A*02, B*51 Predicted Immunization of mice; Cytotoxicity assay; Binding assay; CTL assay
RVTGGVFLVDK Pol 368–378 [74] A*11 Predicted Binding assay; CTL assay; Cytotoxicity assay
VTGGVFLVDK Pol 369–378 [74] A*03, A*11 Predicted Binding assay; CTL assay; Cytotoxicity assay
FLVDKNPHNT Pol 374–383 [62] A*0203 Predicted ELISpot; ICS; Binding assay
LVVDFLHQFSR Pol 377–386 [9] A*1101, A*3301, A*6801 Predicted Proliferation assay; Tetramer staining; Binding assay; Immunization of mice; ELISpot; CTL assay; Cytotoxicity assay
SRLVVDFSQF Pol 386–395 [63] B*1301 overlapping ICS; ELISpot
VVDFSQFSR Pol 389–397 [74,91] A*11, A*6801, A*33 Predicted Stabilization assay; ELISpot; Cytotoxicity assay; Binding assay; Immunization of mice; CTL assay
SWPKFAVPNL Pol 392–401 [45] A*2402 Predicted ELISA
WPKFAVPNL Pol 393–401 [45] B*0702 Predicted ELISA
FAVPNLQSL Pol 396–404 [45] A*0201 Predicted ELISA
NLQSLTNLL Pol 411–419 [105,106] A*0201 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; CTL assay
LLSSNLSWL Pol 418–426 [65,105,106] A*0201 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; CTL assay; ELISpot; Tetramer staining
NLSWLSLDV Pol 422–430 [101,105,106] A*0201, A*02 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; CTL assay; ELISpot
LSLDVSAAFY Pol 426–435 [81] A*0101, A*2902, A*3002 Predicted Binding assay; Immunization of mice; ELISpot
HPAAMPHLL Pol 440–448 [74] B*0702 Predicted Binding assay; CTL assay; Cytotoxicity assay
HLLVGSSGL Pol 446–454 [105,106] A*0201 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; CTL assay
GLPRYVARL Pol 453–461 [65,71,73,74,81,92,93,100,101,106,109,110,111] A*0201, A*0202, A*0203, A*02, A*0207 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; CTL assay; ELISpot; Tetramer staining
RIINNQHR Pol 466–473 [9] A*1101 Predicted Proliferation assay; Tetramer staining
RNLYVSLLL Pol 484–492 [85] A*2402 Predicted ELISpot
NLYVSLLLL Pol 485–493 [65,106] A*0201, A*02 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; CTL assay; ELISpot; Tetramer staining
KLHLYSHPI Pol 500–508 [45,62,93,101,106] A*0201, A*02, A*0203, B*0801 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; CTL assay; ELISpot; Tetramer staining; ELISA
HLYSHPIIL Pol 502–510 [65,105,112,113,114] A*0201, A*02, A*0203 overlapping Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; Tetramer staining
IPMGVGLSP Pol 504–512 [45] B*0702 Predicted ELISA
ILGFRKIPM Pol 509–517 [45] B*0801 Predicted ELISA
FLLAQFTSAI Pol 524–533 [65,101] A*0201, A*02 Predicted ELISpot; Tetramer staining
LLAQFTSAI Pol 525–533 [65,101,106] A*0201, A*02 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; Tetramer staining
SAICSVVRR Pol 531–539 [74] A*11, A*3301, A*6801 Predicted Binding assay; CTL assay; Cytotoxicity assay
SVVRRAFPH Pol 535–542 [9] A*1101 Predicted Proliferation assay; Tetramer staining
FFPHCLAFSYM Pol 539–550 [81] B*07 Predicted Binding assay; Immunization of mice; ELISpot
FPHCLAFSYM Pol 540–550 [74] B*0702, B*3501, B*51, B*5301, B*5401 Predicted Binding assay; CTL assay; Cytotoxicity assay
YMDDVVLG Pol 549–556 [81] A*0201, A*0202, A*0203, A*0206, A*6802 Predicted Binding assay; Immunization of mice; ELISpot
YMDDVVLGA Pol 549–557 [45,71,72,99,101,114,115,116] A*0201, A*02, A*0101 overlapping Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; CTL assay; ELISA
YMDDVVLGAK Pol 549–558 [74] A*03 Predicted Binding assay; CTL assay; Cytotoxicity assay
FLLSLGIHL Pol 573–581 [71,73,74,81,93,106,108,110,116,117,118,119,120] A*02, A*0201, A*0206, A*0202 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; CTL assay; Tetramer staining
SLNFMGYVI Pol 592–600 [106] A*0201 Predicted Binding assay; Immunization of mice; CTL assay; Cytotoxicity assay
PVNRPIDWK Pol 612–620 [9] A*1101 Predicted Proliferation assay; Tetramer staining
PVNRPIDWK Pol 623–631 [74] A*03, A*11 Predicted Binding assay; CTL assay; Cytotoxicity assay
CGYPALMPLY Pol 638–647 [45] A*2402 Predicted ELISA
GYPALMPLY Pol 639–647 [45] A*2402 Predicted ELISA
YPALMPLYA Pol 651–659 [74] B*0702, B*3501, B*51, B*5401 Predicted Binding assay; CTL assay; Cytotoxicity assay
YPALMPLSA Pol 651–659 [62] B*5401 Predicted ELISpot; ICS; Binding assay
ALMPLYACI Pol 653–661 [71,74,93,106] A*0201, A*0202, A*0203, A*0204, A*0206, A*02 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; CTL assay; Tetramer staining
QAFTFSPTYK Pol 665–674 [74,113] A*03, A*11, A*6801 Predicted Cytotoxicity assay; Binding assay; CTL assay
VFADATPTGW Pol 686–695 [45] A*2402 Predicted ELISA
GLCQVFADA Pol 692–700 [45] A*0201 Predicted ELISA
LPIHTAELL Pol 712–720 [45] B*0702 Predicted ELISA
PLPIHTAEL Pol 722–730 [106] A*0201 Predicted Binding assay; Immunization of mice; CTL assay; Cytotoxicity assay
IIGTDNSVV Pol 744–752 [65] A*0201 Predicted ELISpot assay; Tetramer staining
RKYTSFPWLL Pol 744–753 [45] A*2402 Predicted ELISA
KYTSFPWLLG Pol 745–754 [45] A*2402 Predicted ELISA
GTDNSVVLSR Pol 746–755 [74] A*11 Predicted Binding assay; CTL assay; Cytotoxicity assay
KYTSFPWLL Pol 756–764 [63,81,84,88,93] A*24, A*2301, A*2402 overlapping Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; CTL assay; Tetramer staining; ICS; ELISA
LLGCAANWI Pol 763–771 [65,106] A*0201 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; CTL assay; Tetramer staining
WILRGTSFV Pol 770–778 [65,105] A*0201, A*02 Predicted Immunization of mice; Binding assay; ELISpot; Tetramer staining
ILRGTSFVYV Pol 771–780 [65,71] A*0201, A*02 Predicted Cytotoxicity assay; ELISpot; Tetramer staining
DPSRGRLGL Pol 789–797 [74] B*0702 Predicted Binding assay; CTL assay; Cytotoxicity assay
RLGLSRPLL Pol 794–802 [106] A*0201 Predicted Binding assay; Immunization of mice; CTL assay; Cytotoxicity assay
GLSRPLLRL Pol 796–804 [65] A*02 Predicted ELISpot assay; Tetramer staining
LVYRPTTGR Pol 804–812 [9] A*1101 Predicted Proliferation assay; Tetramer staining
SLYADSPSV Pol 814–822 [65,71,73,90,93,106,114,116] A*0201, A*02 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; CTL assay; Tetramer staining; FACS
FLLTRILTI S 20–28 [66,67,68,77,100,121] A*0201 Predicted ICS; Tetramer staining; Cytotoxicity assay; Degranulation assay
PLGFFPDH S 21–28 [122] A*11 Predicted ELISpot
NLLGWSPQA S 73–81 [63] A*0201, A*0207 overlapping ICS; ELISpot
LTTVPAASLLA S 85–95 [95] A*02 Predicted ELISpot
TTSTGPCK S 115–122 [9] A*1101 Predicted Proliferation assay; Tetramer staining
LLDPRVRGL S 131–139 [75] A*02 Predicted CTL assay
AILSKTGDPV S 160–169 [116] A*02 Predicted Tetramer staining
FLGPLLVLQA S 182–190 [62,63,107] C*0801 overlapping Cytotoxicity assay; Binding assay; ELISpot; Tetramer staining;
VLQAGFFL S 188–195 [62] C*0801 Predicted ELISpot; ICS; Binding assay
VLQAGFFLL S 188–196 [65,73,101,116,123] A*0201, A*02 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; CTL assay; Tetramer staining
SWWTSLNFL S 192–200 [85] A*2402 Predicted ELISpot
FLLTRILTI S 194–202 [54,74,76,81,90,93,94,101,108,111,114,116,119,120,123,124,125,126,127,128] A*0201, A*0202, A*0203, A*0206, A*02 overlapping Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; CTL assay; Tetramer staining; ICS; FACS
IPQSLDSWWTSL S 202–213 [129,130] A*0201, A*02 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot
SILSPFLPLL S 207–216 [131] A*0201 Predicted Binding assay; ELISpot
NILSPFMPLL S 207–216 [131] A*0201 Predicted Binding assay; ELISpot
ILSPFMPLL S 208–216 [131] A*0201 Predicted Binding assay; ELISpot
TLSPFLPLL S 208–216 [131] A*0201 Predicted Binding assay; ELISpot
SWWTSLNFL S 208–216 [84] A*24 Predicted Tetramer staining
FLGGTPVCL S 215–223 [95,116,123,125] A*0201A*02, A*24 Predicted Cytotoxicity assay; Immunization of mice; Binding assay; ELISpot; CTL assay; Tetramer staining
SWLSLLVPF S 226–234 [85] A*2402 Predicted ELISpot
RWMCLRRFII S 236–245 [85] A*2402 Predicted ELISpot
CPGYRWMCL S 243–251 [108] B*07 Predicted Cytotoxicity assay
GYRWMCLRR S 245–253 [91] A*33 Predicted Stabilization assay; ELISpot; Cytotoxicity assay; Immunization of mice
RWMCLRRFII S 247–256 [81] A*2301, A*2402 Predicted Binding assay; Immunization of mice; ELISpot
ILLLCLIFL S 260–268 [73,125] A*0201 Predicted Cytotoxicity assay; Immunization of mice
LLLCLIFLL S 261–268 [72] A*02 Predicted Cytotoxicity assay
LLCLIFLLV S 262–269 [65,115,123] A*0201, A*02 Predicted Stabilization assay; ELISpot; Cytotoxicity assay; Tetramer staining; Immunization of mice
LCLIFLLVL S 263–271 [85] A*2402 Predicted ELISpot
(L)VLLDYQGML S 269/70–278 [75] A*0201 Predicted CTL assay
LLDYQGMLP S 271–279 [123] A*0201 Predicted Immunization of transgenic mice; Cytotoxicity assay; ELISpot; Binding assay
LLDYQGMLPV S 271–280 [72,101,116,125] A*02 Predicted ELISpot; Cytotoxicity assay; Binding assay; Tetramer staining
TSMFPSCCCTK S 305–315 [9] A*1101 Predicted Proliferation assay; Tetramer staining
IPIPSSWAF S 324–332 [74,76,81,108] B*0702, B*3501, B*51, B*5301, A*03, B*07, A*02, B*5101 Predicted ELISpot; Cytotoxicity assay; Immunization of mice; Binding assay; CTL assay
YLWEWASVR S 335–343 [91] A*33 Predicted Stabilization assay; ELISpot; Cytotoxicity assay; Immunization of mice
RFSWLSLLVPF S 343–353 [81] A*2301, A*2402 Predicted Binding assay; Immunization of mice; ELISpot
SWLSLLVPF S 345–353 [84] A*24 Predicted Tetramer staining
WLSLLVPFV S 346–354 [71,72,73,74,75,76,99,105,108,117,118,120,123,132,133] A*02, A*0201, A*0202, A*0203, A*0206, A*0207, A*04, A*6802 Predicted ELISpot; Cytotoxicity assay; Immunization of mice; Binding assay; Tetramer staining
LLVPFVQWFV S 349–358 [93,101,111] A*02 Predicted ICS; Degranulation assay; ELISpot; Tetramer staining
VGLSPTVWL S 358–366 [85] A*2402 Predicted ELISpot
GLSPTVWLS S 359–367 [123] A*0201 Predicted Immunization of transgenic mice; Cytotoxicity assay; ELISpot; Binding assay
GLSPTVWLSV S 359–368 [72,73,90,93,105,111,114,116,124,125,128,130,134] A*02, A*0201, A*0203, A*0207 overlapping Immunization of mice; FACS; CTL assay; ELISpot; Tetramer staining; Degranulation assay
VWLSVIWM S 364–371 [90] A*0201 Predicted Immunization of mice; FACS
(L)SVIWMMWYW S 366/367–375 [62] B*5801 Predicted ELISpot; ICS; Binding assay
SVIWMMWYW S 367–375 [63,107] B*5801 overlapping Tetramer staining; ICS; ELISpot
SIVSPFIPLL S 370–379 [131] A*0201 Predicted Binding assay; ELISpot
ILSPFLPLL S 371–379 [131] A*0201 Predicted Binding assay; ELISpot
MMWYWGPSLY S 371–380 [74] A*03 Predicted Binding assay; CTL assay; Cytotoxicity assay
NILSPFLPLL S 381–390 [131] A*0201 Predicted Binding assay; ELISpot
SILSPFLPLL S 381–390 [77] A*0201 Predicted ICS; Tetramer staining;
SIVSPFIPLL S 381–390 [72,73,116,123] A*02, A*0201 Predicted Immunization of mice; FACS; CTL assay; ELISpot; Tetramer staining
ILSPFLPLL S 382–390 [75,90] A*0201 Predicted Immunization of mice; FACS; CTL assay
IVSPFIPLL S 382–390 [134] A*0201 Predicted ELISA; Cytotoxicity assay
ILRSFIPLL S 382–390 [95] A*02, A*24 Predicted ELISpot
LLPIFFCLWV S 389–398 [101] A*02 Predicted ELISpot
DIDPYKEFGATVELL Core 2–16 [135] DRB1*0401 overlapping Proliferation assay; ICS
IDPYKEFGATVELLS Core 3–17 [135] DRB1*0401 overlapping Proliferation assay; ICS
DPYKEFGATVELLSF Core 4–18 [135] DRB1*0401 overlapping Proliferation assay; ICS
PYKEFGATVELLSFL Core 5–19 [135] DRB1*0401 overlapping Proliferation assay; ICS
YKEFGATVELLSFLP Core 6–20 [135,136] DRB1*0401, DRB1*1202 overlapping ICS; Proliferation assay
KEFGATVELLSFLPS Core 7–21 [135] DRB1*0401 overlapping Proliferation assay; ICS
EFGATVELLSFLPSD Core 8–22 [135] DRB1*0401 overlapping Proliferation assay; ICS
FGATVELLSFLPSDF Core 9–23 [135] DRB1*0401 overlapping Proliferation assay; ICS
GATVELLSFLPSDFF Core 10–24 [135] DRB1*0401 overlapping Proliferation assay; ICS
TVELLSFLPSDFFPS Core 12–26 [135] DRB1*0401 overlapping Proliferation assay; ICS
VELLSFLPSDFFPSV Core 13–27 [135] DRB1*0401 overlapping Proliferation assay; ICS
LLSFLPSDFFPSVRD Core 15–29 [135] DRB1*0401 overlapping Proliferation assay; ICS
LSFLPSDFFPSVRDL Core 16–30 [135] DRB1*0401 overlapping Proliferation assay; ICS
FLPSDFFPSVRD Core 18–29 [137] DPw4, DRB1*07 Predicted Cytotoxicity assay
RDLLDTASALYREALESPEH Core 28–47 [138] DRB1*07, DPw4 overlapping Proliferation assay
ALYREALESPEHCSP Core 36–50 [136] DRB1*1202 overlapping ICS
ALESPEHCSPHHTALRQAIL Core 41–60 [139] DRB1*13 overlapping Proliferation assay
EHCSPHHTALRQAIL Core 46–60 [136] DRB1*0803 overlapping ICS
PHHTALRQAILCWGELMTLA Core 50–69 [81] DRB1*07, DRB1*09, DRB1*11 Predicted Binding assay; Immunization of mice; ELISpot
HHTALRQAILCWGEL Core 51–65 [136] DRB1*1202 overlapping ICS
RQAILCWGELMNLAT Core 56–70 [136] DRB1*0803, DRB1*1202 overlapping ICS
LCWGELMTLATWVGVN Core 60–76 [140] DRB1*0101 Predicted Proliferation assay; ICS; Tetramer staining
MNLATWVGSNLEDPA Core 66–80 [136] DRB1*0803 overlapping ICS
LEDPASRELVVSYVN Core 76–90 [136] DRB1*1202 overlapping ICS
SRELVVSYVNVNMGL Core 81–95 [136] DRB1*0803 overlapping ICS
LEYLVSFGVWIRTPP Core 116–130 [136] DRB1*1202 overlapping ICS
EYLVSFGVWIRTPPA Core 117–131 [138] DRW52, DRB1*06 overlapping Proliferation assay
VSFGVWIRTPPAYRPPNAPI Core 120–139 [81,138] DRB1*01, DRB1*07, DRB1*11, DRB1*12, DRB1*13 overlapping Binding assay; Immunization of mice; ELISpot; Proliferation assay
NAPILSTLPETTVVR Core 136–150 [136] DRB1*0803 overlapping ICS
STLPETTVVRRRGRS Core 141–155 [136] DRB1*1202 overlapping ICS
STLPETTVVRRRGRSPRRRT Core 141–160 [141] DRB1*13 Predicted Proliferation assay; Cytotoxicity assay; ICS
PRRRTPSPRRRRSQS Core 156–170 [136] DRB1*0803 overlapping ICS
PPAYRPPNAPILSTL Core 158–172 [135] DRB1*0101 overlapping Proliferation assay; ICS
PAYRPPNAPIL Core 159–169 [142] DR52, DRw3 overlapping Proliferation assay; Cytotoxicity assay
PSPRRRRSQSPRRRR Core 161–175 [136] DRB1*0803 overlapping ICS
RRSQSPRRRRSQSRE Core 166–180 [136] DRB1*1202 overlapping ICS
YFKDCLFKDWEELGE HBx 111–125 [143] DRB1*1301 overlapping ELISpot; Binding assay; ICS
EIRLKVFVLGGCRHK HBx 126–140 [143] DRB1*0101, DRB1*0401, DRB1*1301, DRB5*0101 overlapping ELISpot; Binding assay; ICS
VFVLGGCRHKLVCAP HBx 131–145 [143] DRB1*1301 overlapping ELISpot; Binding assay; ICS
VGPLTVNEKRRLKLI Pol 96–111 [113] DRB1*0301 Predicted ELISpot; Cytotoxicity assay
RHYLHTLWKAGILYK Pol 145–160 [113] DRB1*0301, DRB1*07, DRB1*08, DRB1*09, DRB1*11, DRB1*12, DRB1*15 Predicted ELISpot; Cytotoxicity assay
ESRLVVDFSQFSRGN Pol 385–400 [113] DRB1*03, DRB1*04 Predicted ELISpot; Cytotoxicity assay
LQSLTNLLSSNLSWL Pol 412–427 [113] DRB1*01, DRB1*04, DRB1*07, DRB1*11, DRB1*12, DRB1*13, DRB1*15 Predicted ELISpot; Cytotoxicity assay
SSNLSWLSLDVSAAF Pol 420–435 [113] DRB1*01, DRB1*03, DRB1*04, DRB1*13 Predicted ELISpot; Cytotoxicity assay
LHLYSHPIILGFRKI Pol 501–516 [113] DRB1*01, DRB1*04, DRB1*11 Predicted ELISpot; Cytotoxicity assay
PFLLAQFTSAICSVV Pol 525–538 [81] DRB1*01, DRB1*04, DRB1*07, DRB1*08, DRB1*09, DRB1*11, DRB1*15, DRB5*01 Predicted Binding assay; Immunization of mice; ELISpot
KQCFRKLPVNRPIDW Pol 618–633 [81,113] DRB1*01, DRB1*04, DRB1*07, DRB1*13 Predicted Binding assay; Immunization of mice; ELISpot; Cytotoxicity assay
LCQVFADATPTGWGL Pol 649–664 [81] DRB1*03, DRB1*04, DRB1*07 Predicted Binding assay; Immunization of mice; ELISpot
KQAFTFSPTYKAFLC Pol 664–679 [113] DRB1*01, DRB1*04, DRB1*07, DRB1*08, DRB1*09, DRB1*11, DRB1*13, DRB1*15 Predicted ELISpot; Cytotoxicity assay
AANWILRGTSFVYVP Pol 676–691 [81] DRB1*07, DRB1*08, DRB1*09, DRB1*12, DRB1*13, DRB1*15 Predicted Binding assay; Immunization of mice; ELISpot
LCQVFADATPTGWGL Pol 694–709 [113] DRB1*03, DRB1*04 Predicted ELISpot; Cytotoxicity assay
AANWILRGTSFVYVP Pol 767–782 [113] DRB1*01, DRB1*07, DRB1*08, DRB1*09, DRB1*13, DRB1*15 Predicted ELISpot; Cytotoxicity assay
GTSFVYVPSALNPAD Pol 774–789 [81] DRB1*01, DRB1*04, DRB1*07, DRB1*08, DRB1*09, DRB1*11, DRB1*15, DRB5*01 Predicted Binding assay; Immunization of mice; ELISpot
AGFFLLTRILTIPQS S 17–31 [144] DRB1*07, DRB1*08, DRB1*11, DRB1*13 Predicted ELISpot; Proliferation assay
GFFPDHQLDPAF S 23–33 [145] DRB1*0405 Predicted Binding assay; FASC
TSLNFLGGSPVCLGQ S 37–51 [144] DRB1*01 Predicted ELISpot; Proliferation assay
GAFGPGFTPPHG S 61–72 [145] DRB1*0405 Predicted Binding assay; FASC
PICPGYRWMCLRRFI S 67–81 [144] DRB1*08, DRB1*11, DRB1*13 Predicted ELISpot; Proliferation assay
GWSPQAQGVLTT S 76–87 [145] DRB1*0405 Predicted ELISpot; Proliferation assay
MQWNSTTFHQTLQDPRVRGL S 109–134 [47] DRB1*01 Predicted Immunization of mice; Proliferation assay; ELISpot
TTFHQTLQDPRVRGL S 114–128 [47] DRB1*01 Predicted Immunization of mice; Proliferation assay; ELISpot
MQWNSTAFHQTLQDP S 109–123 [146] DRB1*02 Predicted Proliferation assay; Cytotoxicity assay
STLPETTVVRRRGRSPRRRT S 141–160 [139] DRB1*13 overlapping Proliferation assay
WASVRFSWLSLL S 165–176 [147] DRB1*11, DRB1*14 Predicted CTL assay; Proliferation assay
VPFVQWFVGLSPTVW S 177–191 [144] DRB1*11 Predicted ELISpot; Proliferation assay
QAGFFLLTRILTIPQS S 179–194 [47] DRB1*01 Predicted Immunization of mice; Proliferation assay; ELISpot
WLSVIWMMWYWGPSL S 191–205 [136] DRB1*1202 overlapping ICS
TSLNFLGGTTVCLGQ S 200–214 [47] DRB1*01 Predicted Immunization of mice; Proliferation assay; ELISpot
GPSLYSIVSPFIPLL S 202–216 [144] DRB1*07 Predicted ELISpot; Proliferation assay
LLPIFFCLWVYI S 215–226 [147] DRB1*07, DRB1*08, DRB1*14 Predicted CTL assay; Proliferation assay
PICPGYRWMCLRRFIIFL S 241–258 [148] DRB1*0201 overlapping Tetramer staining
FLLVLLDYQGMLP S 256–268 [54] DP4 Predicted Immunization of mice; Proliferation assay; ELISpot
WEWASARFSWLSL S 326–338 [54] DP4 Predicted Immunization of mice; Proliferation assay; ELISpot
WLSLLVPFVQWFVGL S 335–349 [149] DRB1*0101 Predicted Immunization of mice; Pentamer staining; ELISpot; ICS; Cytotoxicity assay
SLLVPFVQWFVGLSPTVWLSV S 337–357 [47] DRB1*01 Predicted Immunization of mice; Proliferation assay; ELISpot
SVRFSWLSLLVPFVQWF S 343–357 [148] DRB1*0201 overlapping Tetramer staining
VGLSPTVWLSVI S 347–358 [54] DP4 Predicted Immunization of mice; Proliferation assay; ELISpot
GLSPTVWLSVIW S 348–359 [149] DRB1*0101 Predicted Immunization of mice; Pentamer staining; ELISpot; ICS; Cytotoxicity assay
TVWLSVIWMMWYW S 352–364 [54] DP4 Predicted Immunization of mice; Proliferation assay; ELISpot