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. 2001 Jan;21(1):126–135. doi: 10.1128/MCB.21.1.126-135.2001

FIG. 2.

FIG. 2

Northern analysis of APRT expression in various cell lines. Lane 1, transcript levels in cell line RMP41 (20), which has a single copy of the APRT gene. Lane 2, transcript levels in cell line DELI26 (20), which has a tandem duplication structure but in which the downstream copy of APRT is lacking the promoter and 5′ half of the gene. Absence of a transcript in this strain indicates that no stable transcript is made from the truncated, upstream copy of APRT, which is identical to that in the other strains tested. Lane 3, transcript levels in cell line GSAA5, which carries I-SceI and FRT recognition sites. Lane 4, transcript levels in cell line AK209, which carries an HPRT insert. Lane 5, transcript levels in cell line AK213, which carries a CCCTAA insert. Lane 6, transcript levels in cell line AK775, which carries a TTAGGG insert. Hybridization to GAPDH serves as a loading control.