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. 2022 Feb 15;23(4):2154. doi: 10.3390/ijms23042154

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of FABP6 knockdown on mTOR/Akt and autophagy in BC cells. (A) Western blotting analysis of p-AKT, AKT, p-mTOR, and mTOR after knockdown of FABP6 for 72 h in TSGH-8301 and T24 cells. (B) Western blotting analysis of expression of p62 and LC3B-I/LC3B-II after knockdown of FABP6 for 72 h. Left panel: TSGH-8301 cell line; right panel: T24 cell line. (C) PI3K inhibitor LY294002 (20 μM) or DMSO were co-treated with siFABP6 for 72 hr Cell survival was measured using MTT assays. *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to siNeg group. ###, p < 0.001 compared to siFABP6 group.